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Research Initiation Awards: Configurational Transport Processes in Reactions with Enzymes Bound to Fabricated Tailored Supports

研究启动奖：与结合到定制支撑物上的酶反应中的构型运输过程

基本信息

批准号：
9111034
负责人：
Johannes Nitsche
金额：
$ 6.48万
依托单位：
SUNY at Buffalo
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1991
资助国家：
美国
起止时间：
1991-07-01 至 1993-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9111034&HistoricalAwards=false
关键词：
Research Initiation Awards Configurational Transport

项目摘要

The objective of this project is to investigate the configurational transport processes that underline site- specific reaction with soluble and immobilized enzymes, via theoretical, numerical and experimental techniques. The specific goals are: (1) to further the understanding of confined transitional, rotational and conformational diffusion of macromolecular substrates in fine pores; (2) to develop a predictive theory describing the interplay between these configurational modes of transport and site-specific binding steps fundamental to enzymatic reactions; (3) to work the detailed diffusion-reactions theory evolved into a comprehensive computer-aided methodology for optimization of conversion rates with respect to structural and physicochemical support properties; (4) to aid the development of industrial applications of immobilized enzymes via detailed study of large- substrates binding and other phenomena of practical relevance; and (5) to fabricate porous carriers with tailored microstructures and test them experimentally. This work will furnish an experimentally-supported fundamental transport framework for analyzing site-specific diffusion- reaction processes. Its practical value derives from its quantification of poorly understood pore-scale phenomena that mask intrinsic kinetics, for which improved understanding will empower commercial and biomedical process development.

该项目的目的是通过理论、数值和实验技术，研究与可溶性和固定化酶的位点特异性反应的构型传递过程。具体目标是：(1)进一步理解密闭过渡；大分子底物在细孔中的旋转和构象扩散(2)发展一种预测理论，描述这些构型运输模式与酶促反应基础的位点特异性结合步骤之间的相互作用；(3)将详细的扩散反应理论演变为一种综合的计算机辅助方法，用于优化转化率，并考虑结构和物理化学支持性质；(4)通过对大底物结合和其他实际相关现象的详细研究，帮助固定化酶的工业应用的发展；(5)制作具有定制微结构的多孔载体并进行实验测试。这项工作将为分析特定位点的扩散-反应过程提供一个实验支持的基本输运框架。它的实用价值来自于它对掩盖内在动力学的不太了解的孔隙尺度现象的量化，对此的更好理解将赋予商业和生物医学工艺开发权力。