CORE--SOLID PHASE PEPTIDE SYNTHESIS AND HPLC/CZE/CD

核心--固相肽合成及HPLC/CZE/CD

• 批准号: 6105168
• 负责人: JEAN E RIVIER
• 金额: $ 21.48万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6105168
• 关键词: biomedical facility; capillary electrophoresis; circular dichroism; high performance liquid chromatography; neuropeptides; nuclear magnetic resonance spectroscopy; peptide chemical synthesis; protein purification; protein structure; zonal electrophoresis

Description: (Taken directly from the application). Using the (SPPS), Core D will synthesize, each year, a total of approximately 10-20 Core D will synthesize each year a total of approximately 10-20 peptides. Using the latest techniques for the preparative (large scale: mg to gram latest techniques for the preparative (large scale: mg to gram large quantities (10 to 100mg) of purified peptides to support functional large quantities (10 to 100 mg) of purified peptides to support (See Projects I, II, V, VI). Using the latest analytical techniques we polyclonal antibodies. Using the latest analytical techniques we will proteins including that of peptides to be used clinically (Projects I and including that of peptides to be used clinically. Determination of performance liquid chromatography (RP-HPLC), ion exchange chromatography chromatography (RP-HPLC) ion exchange chromatography (IEC) on fast chromatography (SEC) using HPLC when appropriate, and capillary zone (SEC) using HPLC when appropriate and capillary zone electrophoresis (AAA). Mass spectroscopic analysis and Edman degradation (when necessary) spectroscopic analysis and Edman degradation (when necessary) will be under the aegis of this core will support most projects (except Project II) of this core will support most projects (except Project II) while to purify protein on large scale (> 100 mg). Circular dichroism studies to purify protein on large scale (greater than 100 mg). Finally we Finally, we intend to use some of the resources of this core to develop new analytical techniques and explore new applications. This core will take advantage of the PI's experience (10% effort) and of that of that of his technical assistants as well as of his fully functional analytical laboratory. Laboratory space equipped with hoods and other small equipment equipment is available. Major instruments available include a 60 MHz NMR Beckman P/ACE model 2050 CZE, a Perkin Elmer AAA, an Aviv circular circular dichroism (CD) spectrometer model 62S two Hewlett Packard HPLC with diode array detectors and several other analytical and preparative preparative HPLCs. The Core Leader will chair the users committee priorities. It is expected that Core D will be able to operate on a first able to operate on a first come first served basis. In cases where determined, the decision reached by the majority of the users' committee of the users committee (excluding the chair) will be implemented. Solid including purification will be carried out by L. Cervini (25% effort). L. Cervini (25 percent effort). Quality control cmd method development carried out by C. Miller (20 % effort) and J. Dykert (30% effort). J. effort) and J. Dykert (30 percent effort). J. Dykert will also run the carried out by D. Pantoja. Pantoja.

描述：（直接从应用程序中获取）。使用 核心项目D每年将综合总共约10-20个 核心D每年将合成总共约10-20种肽。 使用最新的制备技术（大规模：mg至g 最新的制备技术（大规模：mg至g 大量（10至100 mg）纯化肽，以支持功能性 大量（10至100 mg）纯化的肽以支持 (See项目一、二、五、六）。 利用最新的分析技术， 多克隆抗体。使用最新的分析技术，我们将 蛋白质，包括临床上使用的肽的蛋白质（项目I和 包括临床上使用的肽。测定 高效液相色谱法（RP-HPLC）、离子交换色谱法 快速离子交换色谱法（IEC） 适当时，使用HPLC和毛细管区带色谱法（SEC） (SEC)适当时使用HPLC和毛细管区带电泳 （AAA）。 质谱分析和Edman降解（必要时） 光谱分析和Edman降解（必要时）将 在该核心项目的支持下，将支持大多数项目（项目II除外） 这一核心将支持大多数项目（项目II除外）， 以大规模纯化蛋白质（> 100 mg）。 圆二色性研究 以大规模纯化蛋白质（大于100 mg）。最后我们 最后，我们打算利用这个核心的一些资源来开发新的 分析技术和探索新的应用。这个核心将 PI的经验优势（10%的努力）和他的经验优势 技术助理以及他的全面职能分析 实验室 实验室空间，配备通风橱和其他小型设备 设备可用。可用的主要仪器包括60 MHz NMR Beckman P/ACE型号2050 CZE，Perkin Elmer AAA，Aviv圆形 圆二色（CD）光谱仪型号62 S两惠普HPLC 与二极管阵列检测器和其他几个分析和制备 制备型HPLC。核心领导人将主持用户委员会 优先事项 预计核心D将能够在第一个 能够在先到先得的基础上运作。的情况下 决定，由用户委员会的大多数人达成的决定 用户委员会（不包括主席）的职能将得到落实。固体 包括纯化将由L. Cervini（25%的努力）。 L. Cervini（25%的努力）。质量控制CMD方法开发 由C.米勒（20%的努力）和J. Dykert（30%的努力）。 J. J. Dykert（30%的努力）。J. Dykert还将负责 由D.潘托哈 潘托哈