Analysis of Neuropeptides Using Capillary Electrophoresis with Fluorescence Detection

使用毛细管电泳和荧光检测分析神经肽

• 批准号: 9115313
• 负责人: Jonathan Sweedler
• 金额: $ 3.63万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9115313&HistoricalAwards=false
• 关键词: Analysis; Neuropeptides; Using; Capillary; Electrophoresis

This award is made as the starter grant increment of Dr. Jonathan Sweedler's Postdoctoral Fellowship in Chemistry Award in support of his research at the University of Illinois. The theme of the research is the analysis of neuropeptides using capillary zone electrophoresis with multichannel fluoresence detection. The research will develop new analytical methodology, capillary zone electrophoresis with laser-induced fluorescence detection, to assay neuropeptides released from single neurons in the marine mollusk Aplysia californica. Experiments will perform measurements in the low zeptomole range. This represents a thousand-fold increase in sensitivity over conventional sampling techniques. %%% The development of this ultrasensitive technique will give the cellular biochemist the ability to unravel the complex nature of peptides as neurotransmitters.

该奖项是作为博士的启动补助金增量。 Jonathan Sweedler化学博士后奖学金 以支持他在伊利诺伊大学的研究的 该研究的主题是使用神经肽分析 多通道荧光毛细管区带电泳 侦测该研究将开发新的分析 方法学，激光诱导毛细管区带电泳 荧光检测，以测定从 在海洋软体动物加州滨对虾中的单个神经元。 实验将在低zeptomole条件下进行测量 范围这意味着灵敏度提高了千倍 传统的采样技术。 %%% 这种超灵敏技术的发展将使 细胞生物化学家有能力解开 作为神经递质的肽。