Release of Symbiotic Dinoflagellates from Coral Reef Anthozoans: Effect of Elevated Temperature, Mode of Release,Cellular Mechanisms

珊瑚礁珊瑚虫中共生甲藻的释放：升高温度的影响、释放方式、细胞机制

• 批准号: 9115834
• 负责人: Leonard Muscatine
• 金额: $ 25万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-01-01 至 1996-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9115834&HistoricalAwards=false
• 关键词: Release; Symbiotic; Dinoflagellates; Coral; Reef

This project will investigate the effect of elevated temperature on release of symbiotic animals (zooxanthellae) by tropical corals and sea anemones. In the field, studies will attempt to determine which taxa are susceptible to elevated temperature-induced release, the time-temperature parameters which evoke release, and the mode of release. In the laboratory, studies will investigate the cellular mechanism by which elevated temperature evokes release of zooxanthellae by host cells, using the sea anemone Aiptasia pulchella and selected reef corals. Immunofluorescence and pharmacological agents will be used to characterize levels of free intracellular calcium, integrity of selected skeletal proteins in the cell, and synthesis of calcium-dependent adhesion proteins. Individual host cells will be obtained by maceration. Cells subjected to elevated temperature will be compared to controls at ambient temperature. The results will indicate in ecological terms the potential impact of elevated temperature on coral reef anthozoans, and in cellular terms how elevated temperature causes cell detachment, loss of zooxanthellae and destabilizaiton of marine symbioses which dominate tropical coral reefs.

本项目将研究温度升高对 热带珊瑚释放共生动物（虫黄藻）， 海葵 在该领域，研究将试图确定 哪些分类群对升高的温度诱导的释放敏感， 引起释放的时间-温度参数，以及模式 释放 在实验室中，研究将调查 细胞机制，通过这种机制，温度升高会引起 虫黄藻的宿主细胞，使用海葵Aiptasia pulchella和选定的珊瑚礁。 免疫荧光和 药理学试剂将用于表征游离 细胞内钙，选择的骨骼蛋白的完整性， 细胞，以及钙依赖性粘附蛋白的合成。 将通过浸渍获得单个宿主细胞。 细胞 将经受升高的温度的受试者与对照组进行比较， 环境温度 从生态学的角度来看， 温度升高对珊瑚礁的潜在影响 珊瑚虫，以及在细胞方面，高温如何导致 细胞脱落，虫黄质的损失和 海洋共生体主宰着热带珊瑚礁。