Path of Carbon in Photosynthesis and Release of Glycerol by Zooxanthellae: A Proposal for a Joint Project with a Russian Colleague

该项目是一项联合研究计划 加州大学洛杉矶分校的莱恩·马斯卡廷医生和卡尔·弗吉尼亚医生比尔， 研究所光生物技术系主任 土壤科学与光合作用，俄罗斯科学院。 这项研究将确定碳流产生的路径 在共生甲藻（已知为， zooxanthropium）在海葵和珊瑚，这是如何 途径被宿主因子改变， 细胞释放甘油。 主机系数现在在 在马斯卡廷的实验室里 碳之路 最初将在体外虫黄嘌呤中使用 经典C-14标记和短期光合作用 卡尔文和本森的技术。 在伴随实验中， 研究人员将同时引入刺胞动物宿主， 因素，并试图检测它所影响的途径的变化， 诱导。 从获得的数据，包括分析的关键 酶活性，一个可验证的假说的机制， 宿主因子对还原性磷酸戊糖的作用 途径和甘油合成将制定和 测试.

This project represents a joint research program between the Dr. Len Muscatine of UCLA and Dr. Karl Va. Bil', Head of the Photobiotechnology Department, Institute of Soil Science and Photosynthesis, Russian Academy of Science. The research will determine the path of carbon flow produced in photosynthesis of symbiotic dinoflagellates (known as, zooxanthellae) in sea anemones and corals and how this pathway is altered by a host factor which induces the algal cells to release glycerol. Host factor is now obtained in pure form in Muscatine's laboratory. The path of carbon will initially be determined in zooxanthellae in vitro using classical C-14 labeling and short-term photosynthesis techniques of Calvin and Benson. In companion experiments, the researchers will concurrently introduce cnidarian host factor and attempt to detect changes in the pathway which it induces. From the data obtained, including analysis of key enzyme activities, a testable hypothesis on the mechanism of action of host factor on the reductive pentose phosphate pathway and glycerol synthesis will be formulated and tested.