U.S.-Japan Cooperative Research (Photoconversion/Photo- synthesis): Development of Photosynthesis in Tobacco Leaves:Influence of Cell Ancestry and Positional Information

美日合作研究（光转换/光合成）：烟叶光合作用的发展：细胞祖先和位置信息的影响

• 批准号: 9117292
• 负责人: Judith Croxdale
• 金额: $ 0.62万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1994-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9117292&HistoricalAwards=false
• 关键词: U.S.; Japan; Cooperative; Research; Photoconversion

This award will support a U.S.-Japan Cooperative Photoconversion/Photosynthesis Research Program project between Professor Judith Croxdale, Department of Botany, University of Wisconsin, and Professor Kenji Omasa, Environmental Plant Science Section, National Institute for Environmental Studies, Tsukuba, Japan. The project involves cooperative experiments to examine whether, in the mesophyll of developing dicot leaves, new cells that arise at multiple sites are coordinated by their position in the developing leaf or by their cell lineage. The question is one of fundamental importance to plant developmental biology. The function of clones in developing leaves will be studied by monitoring photosynthesis. The development of the photochemical apparatus will be determined by repeatedly imaging the kinetics of chlorophyll a fluorescence in the clones. Uniform function within clones throughout their development will indicate regulation by cell ancestry, while heterogeneity within clones will indicate the participation of other influences in the development of cellular function. Clones intersected by vascular tissue will be particularly useful in assessing the contributions of other cell types on mesophyll differentiation. Since the clones created will span the entire period of leaf development, the investigators can determine if there are "windows" during development when ancestry may control function, and other times when positional information may regulate cellular development.

该奖项将支持威斯康星大学植物系Judith Croxdale教授和日本筑波国家环境研究所环境植物科学部Kenji Omasa教授之间的美日合作光转化/光合作用研究计划项目。该项目涉及合作实验，以检验在发育中的双子叶的叶肉中，在多个位置出现的新细胞是否与它们在发育中的叶片中的位置或它们的细胞谱系相协调。这个问题是植物发育生物学的基本问题之一。无性系在叶片发育中的作用将通过监测光合作用来研究。光化学装置的发展将通过重复成像克隆中叶绿素a荧光的动力学来确定。在克隆的整个发育过程中，克隆内的一致功能将表明细胞祖先的调节，而克隆内的异质性将表明其他影响参与细胞功能的发展。由维管组织相交的无性系在评估其他类型细胞对叶肉分化的贡献时将特别有用。由于所创造的克隆将跨越叶片发育的整个时期，研究人员可以确定在发育过程中是否存在祖先可能控制功能的“窗口”，以及其他时间位置信息可能调节细胞发育的时间。