The Infrastructure of Mitochondrial Matrix Proteins

线粒体基质蛋白的基础设施

• 批准号: 9117385
• 负责人: Paul Srere
• 金额: $ 20万
• 依托单位: University of Texas Southwestern Medical Center
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-15 至 1996-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9117385&HistoricalAwards=false
• 关键词: Infrastructure; Mitochondrial; Matrix; Proteins

There is now convincing evidence on many levels that metabolic pathways within the mitochondrion are organized. These pathways include the Krebs TCA cycle, fatty acid oxidation, amino acid metabolism, and the Krebs urea cycle. Three areas concerning molecular organization which are still to be elucidated concern the chemical nature of the specific protein-protein interactions, the metabolic consequences of disrupted metabolons, and the relation of the supramolecular complexes to the overall morphology of the mitochondrion. The specific aims of this research are to: a) determine the binding sites on citrate synthase that interact with its membrane binding protein (citrate transporter?) and with its reaction partners, malate dehydrogenase, pyruvate dehydrogenase complex, thiolase, and aconitase; b) analyze the growth and metabolic pattern of yeast cells containing peroxisomal citrate synthase in their mitochondria; and c) determine whether cristal morphology of yeast mitochondria can be changed by the introduction of a characteristic membrane protein from adrenal mitochondrial inner membranes.

现在在许多层面上有令人信服的证据表明， 组织好了内部的通道。 这些途径 包括三羧酸循环、脂肪酸氧化、氨基酸 代谢和Krebs尿素循环。 三个方面涉及 分子组织仍有待阐明的有关 特定蛋白质-蛋白质相互作用的化学性质， 代谢子被破坏的代谢后果， 超分子复合物的整体形态 你好 本研究的具体目标是：a） 确定柠檬酸合酶上与 其膜结合蛋白（柠檬酸转运蛋白？）并以其 反应伴侣，苹果酸脱氢酶，丙酮酸脱氢酶 复合物、硫解酶和乌头酸酶; B）分析生长， 含过氧化物酶体柠檬酸盐的酵母细胞的代谢模式 合成酶;和c）确定晶体是否 酵母线粒体的形态可以通过引入 肾上腺线粒体的一种特征性膜蛋白 内膜