The Infrastructure of Mitochondrial Matrix Proteins

已经表明，在连续的 Krebs三羧酸循环的酶，并且这些 酶也与线粒体内部的蛋白质结合 膜的 然而，仍然有几个克雷布斯 三羧酸循环相互作用， 证明和几个需要更好的 表征了 有可能这些超分子 复合物不仅具有代谢作用， 在线粒体中的作用。 悬而未决的问题 关于线粒体结构是分子基础， 嵴的形式，这显示出很大的变化， 同样的一个。 该项目将：1）分离和 表征内膜的结合蛋白;和 2)研究嵴形成的分子基础。 细胞不仅仅是酶的“袋子”。 组织 细胞的元素以及细胞本身 有效和经济地使用生命的组成部分。 在 在分子水平上， 代谢顺序允许达到更高的通量 每种中间体的浓度较低。 一 第二个好处是，这样的组织不征税， 细胞的溶剂容量有限。 似乎存在过 一些组织面临一些演变压力， 代谢途径在生物体中以单独的酶形式存在 被认为代表了早期的进化条件， 作为细胞中的多功能酶， 在进化的后期才出现。 本项目将提供 多酶复合物在生物体内作用的信息 细胞膜的形成 *//

It has been shown that interactions exist between sequential enzymes of the Krebs tricarboxylic acid cycle and that these enzymes also bind to proteins in the inner mitochondrial membrane. There still remain, however, several Krebs tricarboxylic acid cycle interactions that are not demonstrated and several which need to be better characterized. It is possible that these supramolecular complexes have not only a metabolic role but also a structural role in the mitochondria. An unresolved problem concerning mitochondrial structure is the molecular basis for the form of cristae, which show great variability within the same mitochondrion. This project will: 1) isolate and characterize the binding proteins for the inner membrane; and 2) investigate the molecular basis of cristae form. Cells are not simply "bags" of enzymes. Organization of the elements of cells, as well as cells themselves, allows efficient and economical use of the components of life. At the molecular level, an organized array of the enzymes of a metabolic sequence allows the attainment of higher fluxes with lower concentrations of each of the intermediates. A second benefit is that such organization does not tax the limited solvent capacity of cells. There seems to have been some evolutionary pressure for organization since some metabolic pathways exist as separate enzymes in organisms believed to represent early evolutionary conditions and exist as multifunctional enzymes in cells which are believed to have arisen later in evolution. This project will provide information in the role of multi-enzyme complexes in the formation of cell membranes.***//