The Infrastructure of Mitochondrial Matrix Proteins

线粒体基质蛋白的基础设施

• 批准号: 8821560
• 负责人: Paul Srere
• 金额: $ 18万
• 依托单位: University of Texas Southwestern Medical Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8821560&HistoricalAwards=false
• 关键词: Infrastructure; Mitochondrial; Matrix; Proteins

It has been shown that interactions exist between sequential enzymes of the Krebs tricarboxylic acid cycle and that these enzymes also bind to proteins in the inner mitochondrial membrane. There still remain, however, several Krebs tricarboxylic acid cycle interactions that are not demonstrated and several which need to be better characterized. It is possible that these supramolecular complexes have not only a metabolic role but also a structural role in the mitochondria. An unresolved problem concerning mitochondrial structure is the molecular basis for the form of cristae, which show great variability within the same mitochondrion. This project will: 1) isolate and characterize the binding proteins for the inner membrane; and 2) investigate the molecular basis of cristae form. Cells are not simply "bags" of enzymes. Organization of the elements of cells, as well as cells themselves, allows efficient and economical use of the components of life. At the molecular level, an organized array of the enzymes of a metabolic sequence allows the attainment of higher fluxes with lower concentrations of each of the intermediates. A second benefit is that such organization does not tax the limited solvent capacity of cells. There seems to have been some evolutionary pressure for organization since some metabolic pathways exist as separate enzymes in organisms believed to represent early evolutionary conditions and exist as multifunctional enzymes in cells which are believed to have arisen later in evolution. This project will provide information in the role of multi-enzyme complexes in the formation of cell membranes.***//

研究表明，克雷布斯三羧酸循环的顺序酶之间存在相互作用，这些酶也与线粒体膜内的蛋白质结合。然而，仍有几个克雷布斯三羧酸循环相互作用尚未得到证实，有几个需要更好地表征。这些超分子复合物可能在线粒体中不仅具有代谢作用，而且具有结构作用。关于线粒体结构的一个尚未解决的问题是嵴形式的分子基础，嵴在同一线粒体内表现出很大的变异性。本项目将：1)分离和表征内膜结合蛋白；2)探讨嵴形态的分子基础。细胞不仅仅是酶的“袋子”。细胞元素的组织，以及细胞本身，允许有效和经济地利用生命的组成部分。在分子水平上，代谢序列的酶的有组织的阵列允许在每种中间体的较低浓度下获得较高的通量。第二个好处是这样的组织不会增加细胞有限的溶剂容量。似乎有一些组织的进化压力，因为一些代谢途径作为单独的酶存在于生物体中，被认为代表了早期的进化条件，而作为多功能酶存在于细胞中，被认为是在进化的后期出现的。该项目将为研究多酶复合物在细胞膜形成中的作用提供信息