Functional Aspects of the Structure of the Sea Urchin Bindin Gene and the Molecular Basis of Its Regulation

海胆结合素基因结构的功能及其调控的分子基础

• 批准号: 9117535
• 负责人: Eric Davidson
• 金额: $ 32万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-01-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9117535&HistoricalAwards=false
• 关键词: Functional; Aspects; Structure; Sea; Urchin

In this application Dr. Davidson proposes to capitalize on recent discoveries made in our lab in respect to the molecular basis of bindin-receptor interaction. The species specificity of fertilization in certain sympatic sea urchin species depends on molecular recognition of the acrosomal bindin protein by egg receptor. Previously he has cloned bindin mRNAs and compared the bindin protein sequences from several species of sea urchin, and we have recently identified specific short regions of the protein that are apparently engaged in contact with the receptor, and that are at least in part responsible for the species specificity of the interaction. Here he proposes a series of new experiments: i, we will generate transgenic S. purpuratus expressing S. franciscanus bindin to determine whether presentation of the bindin sequence per se will promote interspecific fertilization; ii, he will also test in vivo the functional significance of alterations in specific regions of bindin sequence; iii, he will determine the polymorphism of the bindin/receptor interaction in S.franciscanus in order to understand how speciation might have come about; iv, he will carry out studies on the interaction of recombinant mutant bindin fragments with the receptor fragment recently described by Foltz and Lennarz; v, he will investigate incorporation of exogenous bindin (and other) genes in growing spermatocytes in culture, and determine whether transgenic spermatocytes can mature into functional sperm in culture.

在这项申请中，戴维森博士建议利用最近的 我们实验室在分子基础方面的发现 结合-受体相互作用 的种属特异性 某些合鳍海胆的受精作用取决于 卵对顶体结合蛋白的分子识别 受体的 此前，他克隆了结合蛋白mRNA，并将其与 来自几种海胆的结合蛋白序列，和 我们最近发现了这种蛋白质的特定短区域， 显然与受体接触， 至少部分负责物种的特异性， 互动 在这里，他提出了一系列新的实验：我，我们 将产生转基因S. purpuratus表达S.方济各会 结合蛋白，以确定结合蛋白序列的呈现是否 se将促进种间受精; ii，他还将测试 在体内，特异性的改变的功能意义 结合序列的区域; iii，他将确定多态性 的结合蛋白/受体相互作用， 理解物种形成是如何发生的; iv，他将携带 重组突变体结合蛋白相互作用的研究 Foltz最近描述的受体片段 和Lennarz; v，他将研究外源性 培养物中生长的精母细胞中的bindin（和其他）基因，以及 确定转基因精母细胞是否可以成熟为 培养中的功能精子。