Patch-Clamping Studies of Mitochondrial Membranes and Reconstitute Systems

线粒体膜和重构系统的膜片钳研究

• 批准号: 9117658
• 负责人: Henry Tedeschi
• 金额: $ 34.73万
• 依托单位: SUNY at Albany
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-15 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9117658&HistoricalAwards=false
• 关键词: Patch; Clamping; Studies; Mitochondrial; Membranes

The aim of this project is study ion channel activities on the inner and outer membranes of mitochondria. Patch clamping will be used to assess channel activity on native mitochondrial membranes and on isolated liposomal membranes into which mitochondrial channel proteins have been reconstituted. These studies will contribute to our understanding of ion movements in the context of mitochondrial function. The regulation of ion movement across mitochondrial membranes is not well understood, but apparently involves several classes of proteins that operate as pumps or as channels. The focus of this work is on channel proteins that facilitate the passive movement of ions across either the inner or outer membrane of the mitochondrion. An electrophysiological technique known as patch clamping will be employed to study the activity of these proteins either in their native membranes, or in a reconstituted artificial membrane system. Patch clamping will be used to assay the ion flux activities through certain channels under a variety of conditions.

本项目的目的是研究细胞膜上离子通道的活性， 线粒体的内膜和外膜。膜片钳将是 用于评估天然线粒体膜上的通道活性 在分离的脂质体膜上， 通道蛋白已经被重建。这些研究将 有助于我们理解离子运动的背景下， 线粒体功能 线粒体膜离子运动的调节是 不太清楚，但显然涉及几类 作为泵或通道的蛋白质。的重点 工作是在通道蛋白，促进被动运动 离子穿过膜的内膜或外膜， 你好一种叫做贴片的电生理技术 将采用钳夹法研究这些蛋白质的活性 无论是在它们的天然膜中，还是在重组的人工膜中， 膜系统膜片钳将用于测定离子通量 在各种条件下通过一定渠道开展活动。