DNA Structure and Dynamics Sensed by Intrinsic Fluorescence

这项研究的目的是确定DNA中的局部区域如何 在特定的相互作用中经历构象转变 蛋白质。 荧光和吸收光谱将 检测局部和全局构象变化。 述的方法 测量可能是次要成分的DNA， 复合物诱导的DNA和蛋白质构象变化 阵 Norlund博士旨在确定： 1. DNA结构运动和结合的热力学 经合组织 RI-DNA复合物。 2. 识别序列中的时间依赖构象 在卵裂过程中。 3. 用直接法测定了Eco RI与DNA的结合常数。 荧光法 4. 核糖、碱基堆积和局部电荷对 荧光变化。 5. ECO RI识别序列中是否存在扭结 在绑定？ 6. Eco RI与GAA碱基的相互作用是否更强 而不是TTC。 7. 融化的“气泡”是否在不太稳定的碱基周围形成， 建议？ 8. 部分堆叠的碱基是否首先被 EcoRI内切核酸酶。 9. DNA碱基瞬时偏移的幅度和速率。 10. 分布模型是否能更好地解释寿命， 离散指数

The goal of this research is to determine how local regions in DNA undergo conformational transitions during specific interactions with proteins. Fluorescence and absorption spectroscopy will detect both local and global conformational changes. The method measures DNA which may be a minor component and senses conformational changes in both DNA and protein induced by complex formation. Dr. Norlund aims to determine: 1. Thermodynamics of DNA structural motions and binding in the Eco RI-DNA complex. 2. Time-dependent conformation in the recognition sequence duringcleavage. 3. The binding constant of Eco RI to DNA using a direct fluorometric method. 4. Contributions of ribose, base stacking and local charge to fluorescence changes. 5. Whether kinks exist in the Eco RI recognition sequence before binding? 6. Whether Eco RI interaction with GAA bases are stronger than with TTC. 7. Whether melting "bubbles" form around less stable bases as suggested? 8. Whether partially-stacked bases are first recognized by the Eco RI Endonuclease. 9. Amplitudes and rates of DNA base transient excursions. 10. Whether distribution models explain lifetimes better than discrete exponentials.