US-Sweden Cooperative Science: Protein-Nucleic Acid Interactions and Dynamics

美国-瑞典合作科学：蛋白质-核酸相互作用和动力学

• 批准号: 8713453
• 负责人: Thomas Nordlund
• 金额: --
• 依托单位: University of Rochester School of Medicine and Dentistry
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-12-15 至 1989-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8713453&HistoricalAwards=false
• 关键词: US; Sweden; Cooperative; Science; Protein

This award will support collaborative research between Professor Thomas M. Nordlund of the University of Rochester School of Medicine and Dr. Rudolf Rigler of the Karolinska Institute, Stockholm in the field of molecular dynamics. In particular, the influence of molecular motions on the binding and dissolution of DNA by the Eco R1 protein will be studied, both through direct experimentation and using computer simulation. The experiments will use an extremely fast photomultiplier to count the photons emitted by the fluorescence of a molecule adjacent to that whose motions are being studied; this fluorescence occurs during binding or disintegration. Molecular excitation will be performed by both laser and synchrotron radiation; the latter has the advantage of continuous tunability and facilities for measuring the time dependence of the fluorescence emission. The computer simulations will involve calculating correlations among energy functions for the various protein-DNA reactions which arise in the binding and dissolution process. The availability of both laser and synchrotron experimental apparatus and expert staff for the computer simulations makes the Karolinska Institute an ideal location for this collaborative research. This work will provide the U.S. investigator with an opportunity to use equipment and techniques to which he does not ordinarily have access, and to learn specialized techniques for subsequent application at his own institution. In addition, the project will increase our understanding of how DNA is modified by interactions with various proteins, which may lead to numerous practical applications.

该奖项将支持教授托马斯M。罗切斯特大学医学院的诺德隆德和斯德哥尔摩卡罗林斯卡学院的鲁道夫·里格勒博士在分子动力学领域的研究。特别是，通过直接实验和计算机模拟，将研究分子运动对Eco R1蛋白结合和溶解DNA的影响。这些实验将使用一个极快的光电倍增管来计算被研究分子附近的荧光发射的光子;这种荧光发生在结合或分解过程中。分子激发将由激光和同步辐射进行;后者具有连续可调谐性和测量荧光发射的时间依赖性的设施的优点。计算机模拟将涉及计算在结合和溶解过程中出现的各种蛋白质-DNA反应的能量函数之间的相关性。激光和同步加速器实验设备以及计算机模拟专家工作人员的可用性使卡罗林斯卡研究所成为这项合作研究的理想地点。这项工作将使美国调查员有机会使用他通常无法获得的设备和技术，并学习专门技术，以便随后在自己的机构中应用。此外，该项目将增加我们对DNA如何通过与各种蛋白质的相互作用而被修饰的理解，这可能会导致许多实际应用。