Role of Stress Proteins in Protein Assembly

为了应对有害的环境压力，如高温和 病毒感染后，生物体合成一种叫做应激的蛋白质 蛋白质或热休克蛋白。 应激蛋白可能 保护生物体免受压力或援助生物体的影响 从压力中恢复过来 虽然应激蛋白是 在应激反应中，一些应激蛋白被诱导， 组成性表达。 这表明应激蛋白 也参与正常的细胞活动。 应激蛋白 可能在发育、信号传导、受体- 介导的内吞作用和蛋白质易位。 的能力 胁迫蛋白质与其他蛋白质结合表明， 它们可以介导蛋白质-蛋白质相互作用， 蛋白质折叠和组装。 提交的初步证据 这一提议表明，胞质因子促进了 细胞溶质70 K应激蛋白之间复合物的解离 和其他蛋白质。 在研究过程中，我将净化 并描述这个因素。 分离和研究了 解离因子将提供深入了解分子 导致蛋白质正确折叠和组装的事件 vivo.

In response to harmful environmental stresses, such as heat and viral infection, organisms synthesize proteins called stress proteins or heat shock proteins. Stress proteins may either protect organisms from the effects of stress or aid organisms during the recovery from stress. Although stress proteins are induced in response to stress, some stress proteins are constitutively expressed. This suggests that stress proteins also participate in normal cellular activities. Stress proteins may play a role in development, signal transduction, receptor- mediated endocytosis, and protein translocation. The ability of stress proteins to associate with other proteins suggests that they may mediate protein-protein interactions and facilitate protein folding and assembly. Preliminary evidence presented in this proposal suggests that a cytosolic factor facilitates the dissociation of complexes between cytosolic 70K stress proteins and other proteins. During the proposed research I will purify and characterize this factor. The isolation and study of the dissociation factor will provide insight into the molecular events leading to proper folding and assembly of proteins in vivo.