Analysis of Genetic Recombination in Arabidopsis_thaliana
Analysis of Genetic Recombination in Arabidopsis_thaliana
批准号:
9118591
负责人:
David Mount
金额:
$31.5万
依托单位:
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
1992
资助国家:
美国
项目状态:
已结题
起止时间:
1992-01-15 至 1995-06-30
中文摘要
植物同源重组的遗传分析 拟南芥将完成。突变体在以下方面有缺陷 重组或具有升高的重组水平的细胞将 被孤立这些突变体将被绘制和表征, 遗传分析这项建议的第一个直接目标是 确定合适的标记系统,用于快速、准确地测量 减数分裂和有丝分裂重组,以便可以使用这些方法 来识别和描述突变体第二个目标是筛选 现有的一组DNA修复缺陷突变体以及新的 诱变植物,用于具有异常重组的个体 频率.第三个目标是描述这种关系 修复和重组之间的联系 这项工作将建立一个遗传系统, 植物重组的分子分析变得可行。
英文摘要
A genetic analysis of homologous genetic recombination in the plant Arabidopsis thaliana will be done. Mutants which are defective in recombination or which have elevated levels of recombination will be isolated. These mutants will be mapped and characterized by genetic analysis. The first immediate goal of this proposal is to identify suitable marker systems for rapid, accurate measurement of meiotic and mitotic recombination so that these methods may be used to identify and characterize mutants. The second goal is to screen an existing set of DNA repair deficient mutants as well as newly mutagenized plants for individuals with abnormal recombination frequencies. The third goal is to characterize the relationship between repair and recombination in Arabidopsis. This work will establish a genetic system that will make a molecular analysis of recombination in plants become feasible.
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海外基金