Analysis of Genetic Recombination in Arabidopsis_thaliana

对拟南芥同源基因重组进行了遗传分析。重组有缺陷或重组水平升高的突变体将被分离。这些突变体将通过遗传分析进行定位和表征。本提案的第一个直接目标是确定合适的标记系统，用于快速，准确地测量减数分裂和有丝分裂重组，以便这些方法可用于识别和表征突变体。第二个目标是筛选一组现有的DNA修复缺陷突变体以及具有异常重组频率的新突变植物。第三个目标是描述拟南芥修复和重组之间的关系。这项工作将建立一个遗传系统，使植物重组的分子分析成为可能。

A genetic analysis of homologous genetic recombination in the plant Arabidopsis thaliana will be done. Mutants which are defective in recombination or which have elevated levels of recombination will be isolated. These mutants will be mapped and characterized by genetic analysis. The first immediate goal of this proposal is to identify suitable marker systems for rapid, accurate measurement of meiotic and mitotic recombination so that these methods may be used to identify and characterize mutants. The second goal is to screen an existing set of DNA repair deficient mutants as well as newly mutagenized plants for individuals with abnormal recombination frequencies. The third goal is to characterize the relationship between repair and recombination in Arabidopsis. This work will establish a genetic system that will make a molecular analysis of recombination in plants become feasible.