Genetic Analysis of Radiation Response in Plants

植物辐射响应的遗传分析

• 批准号: 9418391
• 负责人: David Mount
• 金额: $ 36.61万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-15 至 1997-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9418391&HistoricalAwards=false
• 关键词: Genetic; Analysis; Radiation; Response; Plants

9418391 Mount Genetic analysis of radiation sensitive mutants of Arabidopsis will be done. The involved genes will be mapped, both physically and genetically. Second site suppressors of the uvh1 and uvh6 will be mapped and additional suppressors sought. The genes of interest will be cloned and sequenced. The hypothesis that genetic recombination is an important mechanism of radiation resistance in plants will be tested by determining frequencies of spontaneous mitotic and meiotic recombination in two radiation sensitive mutants, uvh1 and rad3 by genetic crosses. The type of UV damage repair will be analyzed in radiation sensitive mutants in various ways. %%% Very little is known about mechanisms for repair of DNA damage in plants. They are constantly exposed to ultraviolet light in sunlight which damages their DNA and it is known that they have unique mechanisms for avoiding and repairing such damage. A proven genetic approach will be taken to continue an investigation of DNA repair in Arabidopsis, a model plant. The DNA repair genes from this organism will be mapped, cloned, sequenced and analyzed for biochemical function using the modern tools of molecular genetics. ***

拟南芥辐射敏感突变体的遗传分析即将开始。相关的基因将被绘制出来，包括物理上的和基因上的。将绘制uvh1和uvh6的第二位点抑制子，并寻找其他抑制子。将对感兴趣的基因进行克隆和测序。通过遗传杂交测定两个辐射敏感突变体uvh1和rad3的自发有丝分裂和减数分裂重组频率，验证遗传重组是植物抗辐射重要机制的假设。本文将从不同角度分析辐射敏感突变体的紫外线损伤修复类型。我们对植物DNA损伤的修复机制知之甚少。它们经常暴露在阳光中的紫外线下，这会损害它们的DNA，众所周知，它们有独特的机制来避免和修复这种损害。一种已证实的遗传方法将被用于继续研究模式植物拟南芥的DNA修复。DNA修复基因将被绘制、克隆、测序，并利用现代分子遗传学工具分析其生化功能。＊＊＊