Genetic Analysis of Radiation Response in Plants

9418391将对拟南芥辐射敏感突变体进行挂载遗传分析。涉及的基因将被绘制成物理和基因的图谱。第二，将绘制uvh1和uvh6的位点抑制子，并寻找其他抑制子。感兴趣的基因将被克隆和测序。通过对两个辐射敏感突变体uvh1和Rad3的自发有丝分裂和减数分裂重组频率的测定，验证了遗传重组是植物抗辐射的重要机制的假说。紫外线损伤修复的类型将以各种方式分析辐射敏感突变体。对植物DNA损伤的修复机制知之甚少。它们经常暴露在阳光下的紫外线下，破坏它们的DNA，众所周知，它们有独特的机制来避免和修复这种损伤。将采用一种经过验证的遗传方法来继续对模式植物拟南芥的DNA修复进行研究。这种生物的DNA修复基因将被绘制、克隆、测序，并利用现代分子遗传学工具进行生化功能分析。***

9418391 Mount Genetic analysis of radiation sensitive mutants of Arabidopsis will be done. The involved genes will be mapped, both physically and genetically. Second site suppressors of the uvh1 and uvh6 will be mapped and additional suppressors sought. The genes of interest will be cloned and sequenced. The hypothesis that genetic recombination is an important mechanism of radiation resistance in plants will be tested by determining frequencies of spontaneous mitotic and meiotic recombination in two radiation sensitive mutants, uvh1 and rad3 by genetic crosses. The type of UV damage repair will be analyzed in radiation sensitive mutants in various ways. %%% Very little is known about mechanisms for repair of DNA damage in plants. They are constantly exposed to ultraviolet light in sunlight which damages their DNA and it is known that they have unique mechanisms for avoiding and repairing such damage. A proven genetic approach will be taken to continue an investigation of DNA repair in Arabidopsis, a model plant. The DNA repair genes from this organism will be mapped, cloned, sequenced and analyzed for biochemical function using the modern tools of molecular genetics. ***