Regulation of Expression of the Calmodulin-Related TCH Genesin Plants and Cultured Cells

该实验室的长期目标是阐明 分子机制和参与的功能成分 植物基因表达对环境胁迫的响应 刺激。理解这些感觉和反应途径是 对于理解植物的动态本质至关重要 发展5个基因的表达水平，TCH基因， 拟南芥，是迅速和急剧增加，以响应 不同的刺激，如雨，风，触摸，热休克和 山中贼易TCH基因中的三个编码钙调素相关的 蛋白质，提示钙离子和钙调蛋白在 信号转导途径 这项工作的重点是表征监管的 钙调素相关TCH基因在植物和培养细胞中的表达 对各种环境刺激的反应。第一，转录 起始位点、内含子/外显子边界和多聚腺苷酸化位点 将被定义，全长cDNA序列将被 测定钙调素相关的TCH 2和3基因。 随后，TCH 2的组织和发育特异性 和3 mRNA在植物中的积累将被检查， 强调比较答复的特点， 不同的刺激习惯化、脱敏的过程 并将使用TCH mRNA积累研究回收率， 化验此外，TCH 2和3基因的调控 机械应力、热休克和钙离子的表达 使用培养的根细胞， 均质且易于操作。由于普遍的作用， 真核细胞中钙离子第二信使的研究 钙离子编码基因表达的调节 受体可能与各种植物有关， 动物细胞将细胞外刺激转化为钙 信号.

The long-term objective of this laboratory is to elucidate the molecular mechanisms and the functional components involved in the regulation of plant gene expression in response to environmental stimuli. Comprehension of these sensory and response pathways is essential for understanding the dynamic nature of plant development. The expression levels of five genes, the TCH genes of Arabidopsis, are rapidly and sharply increased in response to diverse stimuli, such as rain, wind, touch, heat shock and darkness. Three of the TCH genes encode calmodulin-related proteins, suggesting roles for calcium ion and calmodulin in the signal transduction pathways. The focus of this work is to characterize the regulation of the calmoduln-related TCH genes in plants and cultured cells in response to diverse environmental stimuli. First, the transcription initiation sites, intron/exon boundaries and polyadenylation sites will be defined and the full-length cDNA sequences will be determined for the calmodulin-related TCH2 and 3 genes. Subsequently, the tissue- and developmental-specificity of TCH 2 and 3 mRNA accumulation in plants will be examined, with the emphasis on comparing the characteristics of the responses to different stimuli. The processes of habituation, desensitization and recovery will be investigated using TCH mRNA accumulation as an assay. Additionally, the regulation of TCH 2 and 3 gene expression by mechanical stress, heat shock and calcium ion will be addressed using cultured root cells that are relatively homogeneous and easily manipulated. Because of the universal role of calcium ion a second messenger in eukaryotic cells, elucidation of the regulation of expression of genes that encode calcium ion receptors will likely be relevant for the great variety of plant and animal cells that transduce extracellular stimuli into calcium signals.