Regulation of gone expression by calmodulin-dependent protein kinase II

钙调蛋白依赖性蛋白激酶 II 对 gone 表达的调节

• 批准号: 05454172
• 负责人: YAMAUCHI Takashi
• 金额: $ 4.93万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454172/
• 关键词: protein kinase; calmodulin; central nervous system; gene expression; cultured neuronal cell; カムキナーゼII; リン酸化; α,βアイソフォーム; ATP; 神経芽細胞

Protein phosphorylation is involved in the regulation of many aspects of neuronal function. The study of protein kinases and their specific substrates should continue to provide important information relevant to the molecular mechanisms involved in the regulation of neuronal function. Ca2+plays important roles through the action of calmodulin-dependent protein kinases, by stimulating phosphorylation of a number of endogenous proteins in the brain. Ca_<2+>/calmodulin-dependent protein kinase II (CaM kinase II) is one of the most abundant protein kinases in the brain and plays important roles on neuronal signal transduction. In this study, we investigated the followings ; (i) regulation of the expression of CaM kinase II in cultured hippocampus cells, (ii) structure of CaM kinase II gene, (iii) activation of CaM kinase II in the post synaptic density, (iv) role of CaM kinase II on the differentiation of P19 teratocarcinoma cell, (v) developmental changes in the level of CaM kinase II and its substrate proteins.

蛋白质磷酸化参与调节神经元功能的许多方面。蛋白激酶及其特异性底物的研究将继续为神经元功能调节的分子机制提供重要信息。Ca 2+通过钙调蛋白依赖性蛋白激酶的作用，通过刺激脑中许多内源性蛋白质的磷酸化而发挥重要作用。钙离子/钙调素依赖性蛋白激酶II（CaM kinase II）是脑内含量最丰富的蛋白激酶之一，在神经元信号转导中起重要作用。本研究探讨了海马细胞中CaM激酶II表达的调控，CaM激酶II基因的结构，CaM激酶II在突触后致密区的激活，CaM激酶II在P19畸胎瘤细胞分化中的作用，CaM激酶II及其底物蛋白水平的发育变化。

项目成果

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