Regulation of gone expression by calmodulin-dependent protein kinase II

蛋白质磷酸化参与神经元功能的许多方面的调节。对蛋白激酶及其特定底物的研究将继续为神经元功能调节的分子机制提供重要信息。Ca2+通过钙调素依赖性蛋白激酶的作用，通过刺激大脑中许多内源性蛋白的磷酸化发挥重要作用。Ca_<2+>/calmodulin-dependent protein kinase II （CaM kinase II）是脑内最丰富的蛋白激酶之一，在神经元信号转导中起重要作用。在本研究中，我们调查了以下内容；(i) CaM激酶II在培养海马细胞中的表达调控，（II） CaM激酶II基因的结构，（iii） CaM激酶II在突触后密度中的激活，（iv） CaM激酶II在P19畸形瘤细胞分化中的作用，(v) CaM激酶II及其底物蛋白水平的发育变化。

Protein phosphorylation is involved in the regulation of many aspects of neuronal function. The study of protein kinases and their specific substrates should continue to provide important information relevant to the molecular mechanisms involved in the regulation of neuronal function. Ca2+plays important roles through the action of calmodulin-dependent protein kinases, by stimulating phosphorylation of a number of endogenous proteins in the brain. Ca_<2+>/calmodulin-dependent protein kinase II (CaM kinase II) is one of the most abundant protein kinases in the brain and plays important roles on neuronal signal transduction. In this study, we investigated the followings ; (i) regulation of the expression of CaM kinase II in cultured hippocampus cells, (ii) structure of CaM kinase II gene, (iii) activation of CaM kinase II in the post synaptic density, (iv) role of CaM kinase II on the differentiation of P19 teratocarcinoma cell, (v) developmental changes in the level of CaM kinase II and its substrate proteins.