Genetic characterization and cloning of the barley M1-a locus

大麦 M1-a 基因座的遗传特征和克隆

• 批准号: 9119107
• 负责人: Shauna Somerville
• 金额: $ 21万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9119107&HistoricalAwards=false
• 关键词: Genetic; characterization; cloning; barley; M1

The powdery mildew disease of barley, caused by the obligate fungal pathogen Erysiphe graminis f. sp. hordei, offers several advantages for the study of host genes mediating resistance. This disease has been studied intensively for more than 50 years; thus, there is an extensive base of physiological data and genetic materials available that is unmatched by most other host/pathogen systems. The barley M1-a locus is of particular interest in that more than 30 resistance alleles have been identified at this locus. By screening 118,000 M2 seedlings, I have isolated 41 partially or fully susceptible mutants. Complementation analysis of these new alleles will be used to determine the genetic structure of the M1-a locus, which may represent either a complex locus or cluster of tightly linked genes. Also, the mutants will be exploited in subtractive hybridization experiments to isolate M1-a cDNA clones. No such disease resistance gene has been cloned from any plant. Analysis of the M1-a clones will provide information about the nature of the allelic variation at the M1-a locus and about the biochemical role of the M1-a gene product in mediating disease resistance.***//

大麦白粉病，由专性真菌病原菌白粉菌 f 引起。 sp。 hordei 为研究介导抗性的宿主基因提供了多种优势。 人们对这种疾病进行了 50 多年的深入研究；因此，我们拥有广泛的生理数据和遗传物质基础，这是大多数其他宿主/病原体系统无法比拟的。 大麦 M1-a 基因座特别令人感兴趣，因为已在该基因座鉴定了 30 多个抗性等位基因。 通过筛选118,000株M2幼苗，我分离出41个部分或完全敏感的突变体。 这些新等位基因的互补分析将用于确定 M1-a 基因座的遗传结构，该基因座可能代表一个复杂的基因座或紧密连锁的基因簇。 此外，突变体将用于消减杂交实验以分离 M1-a cDNA 克隆。 目前还没有从任何植物中克隆出这样的抗病基因。 对 M1-a 克隆的分析将提供有关 M1-a 基因座等位基因变异的性质以及 M1-a 基因产物在介导抗病性中的生化作用的信息。***//