Role of the Metalloproteinase Stromelysin in Axon Invasiveness

金属蛋白酶溶基质素在轴突侵袭中的作用

• 批准号: 9119397
• 负责人: Gary Ciment
• 金额: $ 18.91万
• 依托单位: Oregon Health & Science University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-15 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9119397&HistoricalAwards=false
• 关键词: Role; Metalloproteinase; Stromelysin; Axon; Invasiveness

Stromelysins are a family of secreted metalloproteinases that degrade various components of the extracellular matrix. Recent evidence suggests that the secretion of stromelysins from nerve cells may play a role in axonal growth. To address this issue, the neuronal-like clonal cell line, PC12, will be used in a novel in vitro assay to study the effect of experimentally manipulated stromelysin levels on neurite invasiveness of basal lamina. The possible release of stromelysin from PC12 growth cones will also be studied using immunocytochemistry and in situ substrate degradation. In addition, possible tissue-specific and developmental stage-specific expression of stromelysin mRNA will be studied in the embryonic rat. These studies promise to enhance our understanding of molecular mechanisms involved in the growth of axons.

基质溶解素是分泌型金属蛋白酶家族， 降解细胞外基质的各种成分。 最近 有证据表明，从神经分泌的溶基质素 细胞可能在轴突生长中起作用。 为了解决这个问题， 神经元样克隆细胞系PC12将被用于一种新的 体外试验，以研究实验操作的 基质分解素水平对基底膜神经突侵袭的影响。的 从PC12生长锥释放溶基质素的可能性也将 使用免疫细胞化学和原位底物研究 降解 此外，可能的组织特异性和 基质分解素mRNA的发育阶段特异性表达将是 在胚胎大鼠中进行了研究。 这些研究有望提高我们的 了解参与生长的分子机制， 轴突