Luminescence Spectroelectrochemistry of Polynucleotide Complexes

这个项目是在分析和表面化学的一般领域和生物电化学的子领域。在这项为期六个月的标准资助期间，钱伯斯教授和他的学生将采用改良电极和激光诱导荧光光谱电化学技术来研究有机离子（如乙啶）释放到溶液中的过程，以及这些离子随后与双链DNA分子的相互作用。所产生的荧光DNA复合物的分析效用也将进行评估。本研究建立在钱伯斯博士之前获得的美国国家科学基金会资助（CHE-8718057）的基础上，为痕量多核苷酸的检测提供了灵敏的方法，并研究了它们在溶液中的质量传输特性。

This project is in the general area of analytical and surface chemistry and in the subfield of bioelectrochemistry. During the tenure of this six-month standard grant, Professor Chambers and his students will employ modified electrode and laser induced fluorescence spectroelectrochemistry techniques to study the release into solution of organic ions such as ethidium and the ensuing interactions of these ions with double stranded DNA molecules. The analytical utility of the resulting fluorescent DNA complexes will also be evaluated. %%% This research builds upon results obtained under Dr. Chambers' previous NSF grant, CHE-8718057, to provide sensitive methods for the detection of trace amounts of polynucleotides and to study their mass transport properties in solution.