Electrochemistry of Polynucleotides

多核苷酸的电化学

• 批准号: 9220939
• 负责人: James Chambers
• 金额: $ 25.07万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-15 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9220939&HistoricalAwards=false
• 关键词: Electrochemistry; Polynucleotides

This project is in the general area of analytical and surface chemistry with an emphasis on bioelectrochemistry. During the tenure of this three-year grant, Professor Chambers will investigate the possibility of electrochemical communication with polynucleotides or, more specifically, the purine and pyrimidine groups buried within polynucleotides. The experimental techniques will include the use of modified electrodes and laser-induced fluorescence spectroelectro- chemistry. Dr Chambers will design an organic salt electrode that responds to DNA through intercalation or hydrogen-bonding interactions. He will also develop electrochemical tags for DNA and other polynucleotides similar to fluorescent tags currently used for spectroscopic investigations. %%% Professor Chambers and his students will investigate the possibility of electrochemical communication with DNA and other polynucleotides by developing novel, modified electrodes and electrochemical tags similar to spectroscopic tags in common use today. The proposed method for the analysis of DNA and polynucleotides may give rise to bioanalytical techniques with broad applications in clinical chemistry and biotechnology.

这个项目是在一般地区的分析和表面 重点是生物电化学。 期间 在这三年的任期内，钱伯斯教授将 研究电化学通讯的可能性 用多核苷酸，或者更具体地，嘌呤， 埋在多核苷酸内的嘧啶基团。 的 实验技术将包括使用修改后的 电极和激光诱导荧光光谱 化学. 钱伯斯博士将设计一种有机盐电极 通过嵌入或氢键与DNA反应 交互. 他还将开发电化学标签， DNA和其他类似于荧光标记的多核苷酸 目前用于光谱研究。 %%% 钱伯斯教授和他的学生将调查 与DNA和其他生物进行电化学通信的可能性 通过开发新的、修饰的电极和 与光谱标记相似的电化学标记 今天使用。 所提出的用于分析DNA和 多核苷酸可以产生生物分析技术， 广泛应用于临床化学和生物技术。