Electrochemistry of Polynucleotides

这个项目是分析化学和表面化学的一般领域，重点是生物电化学。在这项为期三年的资助期间，钱伯斯教授将研究与多核苷酸或更具体地说，多核苷酸中隐藏的嘌呤和嘧啶基团的电化学通信的可能性。实验技术将包括使用修饰电极和激光诱导荧光光谱电化学。钱伯斯博士将设计一种有机盐电极，通过插入或氢键相互作用对DNA做出反应。他还将开发DNA和其他多核苷酸的电化学标签，类似于目前用于光谱研究的荧光标签。钱伯斯教授和他的学生将研究与DNA和其他多核苷酸进行电化学通信的可能性，方法是开发新型的、改良的电极和电化学标签，类似于目前常用的光谱标签。所提出的分析DNA和多核苷酸的方法可能会在临床化学和生物技术中产生广泛应用的生物分析技术。

This project is in the general area of analytical and surface chemistry with an emphasis on bioelectrochemistry. During the tenure of this three-year grant, Professor Chambers will investigate the possibility of electrochemical communication with polynucleotides or, more specifically, the purine and pyrimidine groups buried within polynucleotides. The experimental techniques will include the use of modified electrodes and laser-induced fluorescence spectroelectro- chemistry. Dr Chambers will design an organic salt electrode that responds to DNA through intercalation or hydrogen-bonding interactions. He will also develop electrochemical tags for DNA and other polynucleotides similar to fluorescent tags currently used for spectroscopic investigations. %%% Professor Chambers and his students will investigate the possibility of electrochemical communication with DNA and other polynucleotides by developing novel, modified electrodes and electrochemical tags similar to spectroscopic tags in common use today. The proposed method for the analysis of DNA and polynucleotides may give rise to bioanalytical techniques with broad applications in clinical chemistry and biotechnology.