Electroactive Oligodeoxynucleotides

电活性寡脱氧核苷酸

• 批准号: 9616994
• 负责人: James Chambers
• 金额: $ 29.4万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-15 至 2000-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9616994&HistoricalAwards=false
• 关键词: Electroactive; Oligodeoxynucleotides

This project, supported in the Analytical and Surface Chemistry Program, focusses on the synthesis of oligodeoxynucleotides that have specific and stable electrochemical signals when immobilized on an electrode surface. Professor James Q. Chambers and his students at the University of Tennessee will use mercuration and coupling of quinones via phosphoramidite linking agents to impart electroactivity to these oligodeoxynucleotides. These immobilized reagents will exhibit shifts in their electrochemical responses upon exposure to DNA molecules with complementary base sequences making a highly selective and sensitive biosensor for specific DNA molecules. Electrochemical, spectroelectrochemical, quartz crystal microbalance, and impedance spectroscopic measurements will be used to characterize these biosensors. Biosensors that can selectively and sensitively detect target DNA molecules will be constructed and characterized in this work by Professor Chambers and his students at the University of Tennessee. Oligodeoxynucleotides will be modified to become electroactive so that when they complex with target DNA on the surface of an electrode, an electroanalytical current can be detected. These biosensors have extensive potential for application in the analysis of polynucleotides with specific base sequences.

该项目得到了分析和表面化学项目的支持，重点是合成固定在电极表面时具有特异性和稳定电化学信号的寡脱氧核苷酸。 詹姆斯·Q教授钱伯斯和他在田纳西大学的学生将使用汞化和通过亚磷酰胺连接剂偶联醌来赋予这些寡脱氧核苷酸电活性。 这些固定化试剂在暴露于具有互补碱基序列的DNA分子时将表现出其电化学响应的变化，从而对特定DNA分子形成高度选择性和灵敏的生物传感器。 电化学，光谱电化学，石英晶体微天平，和阻抗光谱测量将被用来表征这些生物传感器。 可以选择性和灵敏地检测目标DNA分子的生物传感器将由田纳西大学的钱伯斯教授和他的学生在这项工作中构建和表征。 寡脱氧核苷酸将被修饰成具有电活性，使得当它们与电极表面上的靶DNA复合时，可以检测到电分析电流。 这些生物传感器在分析具有特定碱基序列的多核苷酸中具有广泛的应用潜力。