Acquisition of a Liquid Scintillation Counter

本提案中的所有三个主要项目，尽管本质上不同，但都需要使用基本的生化技术——用放射性同位素跟踪代谢功能。其中一项建议是利用实验研究甲状腺激素对脂肪酸合酶基因表达的调控。获得的结果可能使我们能够将某些磷酸化蛋白的消失与甲状腺激素对该基因的转录调节联系起来。提出的另一个项目也研究基因转录的调控，但是通过试图确定甲基化DNA的作用来实现的。第三个项目的重点是了解大鼠肝细胞脂质合成的调控。第四个项目旨在检查各种条件对孤立小动脉cAMP水平的影响。在所有提出的项目中，放射性同位素都以监测或探测的方式使用，因此通过液体闪烁计数对这些测量进行定量是必不可少的。

All three major projects in this proposal, although fundamentally different, require the use of a basic biochemical technique- following metabolic function with radioisotopes. One of the proposals is designed to utilize experiments to study the regulation of fatty acid synthase gene expression by thyroid hormone. Results obtained may possible allow us to correlate the appearance of disappearance of certain phosphoproteins with the transcriptional regulation of this gene by thyroid hormone. Another one of the projects proposed also investigates the regulation of gene transcription but does so by trying to determine the role of methylated DNA. The third project is focused on understanding the regulation of the synthesis of lipids in rat hepatocytes. The forth project is designed to examine the effects of various conditions on the cAMP levels on isolated arterioles. In all projects proposed, radioisotopes are use in a monitoring or detection manner and thus quantitation of these measurements by liquid scintillation counting is essential.