Acquisition of a Liquid Scintillation Counter

这项提案中的所有三个主要项目，尽管从根本上说是不同的，但都需要使用一种基本的生化技术--用放射性同位素跟踪代谢功能。其中一个建议是利用实验来研究甲状腺激素对脂肪酸合成酶基因表达的调节。所获得的结果可能使我们有可能将某些磷蛋白的消失与甲状腺激素对该基因的转录调控联系起来。另一个拟议的项目也调查了基因转录的调节，但通过试图确定甲基化DNA的作用来做到这一点。第三个项目的重点是了解大鼠肝细胞中脂质合成的调节。第四个项目旨在研究不同条件对游离小动脉cAMP水平的影响。在所有拟议的项目中，放射性同位素用于监测或检测，因此，通过液体闪烁计数对这些测量进行量化是必不可少的。

All three major projects in this proposal, although fundamentally different, require the use of a basic biochemical technique- following metabolic function with radioisotopes. One of the proposals is designed to utilize experiments to study the regulation of fatty acid synthase gene expression by thyroid hormone. Results obtained may possible allow us to correlate the appearance of disappearance of certain phosphoproteins with the transcriptional regulation of this gene by thyroid hormone. Another one of the projects proposed also investigates the regulation of gene transcription but does so by trying to determine the role of methylated DNA. The third project is focused on understanding the regulation of the synthesis of lipids in rat hepatocytes. The forth project is designed to examine the effects of various conditions on the cAMP levels on isolated arterioles. In all projects proposed, radioisotopes are use in a monitoring or detection manner and thus quantitation of these measurements by liquid scintillation counting is essential.