Acquisition of a Liquid Scintillation Counter

这三个主要项目，虽然从根本上说， 不同的是，需要使用基本的生物化学技术- 放射性同位素的代谢功能。 之一 建议旨在利用实验来研究 甲状腺对脂肪酸合成酶基因表达的调节 激素. 获得的结果可能使我们能够将 某些磷蛋白的消失， 甲状腺激素对该基因的转录调控。 另一个拟议的项目也调查了 基因转录的调控，但这样做是通过试图 确定甲基化DNA的作用。 第三个项目是 专注于了解合成的调节， 大鼠肝细胞中的脂质。 第四个项目旨在 检查各种条件对cAMP水平的影响， 孤立的小动脉 在所有提出的项目中，放射性同位素是 以监测或检测方式使用，从而定量 通过液体闪烁计数进行这些测量是必要的。

All three major projects in this proposal, although fundamentally different, require the use of a basic biochemical technique- following metabolic function with radioisotopes. One of the proposals is designed to utilize experiments to study the regulation of fatty acid synthase gene expression by thyroid hormone. Results obtained may possible allow us to correlate the appearance of disappearance of certain phosphoproteins with the transcriptional regulation of this gene by thyroid hormone. Another one of the projects proposed also investigates the regulation of gene transcription but does so by trying to determine the role of methylated DNA. The third project is focused on understanding the regulation of the synthesis of lipids in rat hepatocytes. The forth project is designed to examine the effects of various conditions on the cAMP levels on isolated arterioles. In all projects proposed, radioisotopes are use in a monitoring or detection manner and thus quantitation of these measurements by liquid scintillation counting is essential.