Acquisition of a liquid scintillation counter

购买液体闪烁计数器

• 批准号: RTI-2016-00649
• 负责人: Lafontaine, Daniel
• 金额: $ 3.82万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616519
• 关键词: Acquisition; liquid; scintillation; counter

Many important biological processes are directly related to interactions involving DNA, RNA and proteins. Most of these interactions require to monitor very small concentrations of materials, often in the picomolar (10-12 molar) order of magnitude. A wide array of fluorescent approaches is employed to monitor biochemical interactions. However, such fluorescence-based measurements do not provide the sensitivity and ease of use that are still required in a large majority of biochemical assays involving compounds found in very low concentrations. Such limitation is not present when using radiolabeling technology, effectively allowing the measurement of many substances occurring in picomolar concentrations. Interestingly, the non-invasive nature of radiolabels introduces minimal perturbations in studied systems. Furthermore, in addition to detecting the presence of a large array of biological molecules in any model system, the use of radiolabelling has allowed the development of very powerful experimental techniques whereby dual labels are employed to simultaneously detect and distinguish two compounds of interest. The use of radiolabelling has also permitted to develop pulse-chase methods to monitor a substance at various time after its synthesis among a complex mixture of related compounds. Although these assays involve different systems, they nevertheless all depend on the precise detection of radiolabels, which are essential components of these techniques. It is thus vital that our department has access to a high performance scintillation counter allowing us to accurately quantify radiolabels.

许多重要的生物过程与DNA、RNA和蛋白质的相互作用直接相关。这些相互作用中的大多数需要监测非常小的物质浓度，通常在皮摩尔(10-12摩尔)数量级。大量的荧光方法被用来监测生化相互作用。然而，这种基于荧光的测量不能提供大多数生化分析中仍然需要的灵敏度和易用性，这些生化分析涉及的是在非常低浓度下发现的化合物。当使用放射性标记技术时，这种限制不存在，从而有效地允许测量以皮摩尔浓度出现的许多物质。有趣的是，无线电标记的非侵入性在所研究的系统中引入了最小的扰动。此外，除了检测任何模型系统中大量生物分子的存在外，放射性标记的使用还允许开发非常强大的实验技术，从而使用双重标记来同时检测和区分两种感兴趣的化合物。放射性标记的使用还允许开发脉冲追踪法，以便在相关化合物的复杂混合物中合成一种物质后的不同时间进行监测。尽管这些分析涉及不同的系统，但它们都依赖于对放射性标记的准确检测，而放射性标记是这些技术的重要组成部分。因此，至关重要的是，我们的部门能够使用高性能的闪烁计数器，使我们能够准确地量化无线电标记。