Acquisition of Ultracentrifuge, Liquid Scintillation CounterAutoclave, and Water Purification System for Photosensory Research in Microorganisms

请求支持制备超浓缩液 闪烁计数器和高压灭菌器。 本设备 迫切需要光反应的生物化学研究， 两种真核微生物， blakesleeanus和莱茵衣藻Chlamydiumreinhardtii。 在 蓝灯控制着几种反应，包括 向光性，光生长反应，胡萝卜素合成，和 孢子囊梗发育 蓝光光感受器 藻类和许多其他植物和真菌仍然是 鉴定 充分表征的突变体的可用性， 提供了光感受器的生化分离策略， 和其他参与感光传导的分子 流程. 在衣原体中，趋光性和胡萝卜素的合成 使用视紫红质感光器。 然而，这种视紫红质 但仍需纯化到足够的量用于光谱分析 分析. 两个国家的生物化学工作水平不断提高 生物物理学研究小组要求， 在物理大楼内可用，而不是仅在 穿过校园的生物楼 超浓缩咖啡是 细胞分级分离所需的，特别是膜 分馏 将使用液体闪烁计数器 用于受体结合研究和追踪信号通路 使用放射性标记的探针。 将使用高压灭菌器 用于生长介质和设备的灭菌。

Support is requested for a preparative ultracentrifuge, liquid scintillation counter and an, autoclave. This equipment is urgently needed for biochemical studies of photoresponses in two eukaryotic microorganisms, the fungus Phycomyces blakesleeanus and the alga Chlamydomonas reinhardtii. In Phycomyces, blue light controls several response, including phototropism, light growth responses, carotene synthesis, and sporangiophore development. The blue-light photoreceptors in Phycomyces and in many other plants and fungi remain to be identified. The availablity of well characterized mutants, provides a strategy for biochemical isolation of photoreceptors and other molecules involved in photosensory transduction processes. In Chlamydomonas, phototaxis and carotene synthesis employ a rhodopsin photoreceptor. This rhodopsin, however, has yet to be purified in sufficient quantity for spectroscopic analysis. The increasing level of biochemistry work in the two biophysics research groups requires that appropriate equipment be available within the physics building, instead of only in the biology building across campus. The ultracentrifuge is required for cell fractionation, in particular membrane fractionation. The liquid scintillation counter will be used for receptor binding studies and for tracing signaling pathways using radioactively labeled probes. The autoclave will be used for sterilization of rowth media and apparatus.