GLOBEC: Physiological Status of Zooplankton: Utilizing PCR to Rapidly Assess Species-Specific Enzyme Expression

GLOBEC:浮游动物的生理状态:利用 PCR 快速评估物种特异性酶表达

基本信息

  • 批准号:
    9121744
  • 负责人:
  • 金额:
    $ 9.25万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    1992
  • 资助国家:
    美国
  • 起止时间:
    1992-01-15 至 1995-06-30
  • 项目状态:
    已结题

项目摘要

This project offers a unique solution to the problems associated with identifying, sorting and assessing the physiological status of zooplankton. Molecular techniques will be developed that can rapidly identify and quantify the level of expression of physiologically significant genes in the copepod, Calanus finmarchicus. These techniques take advantage of the specificity and sensitivity of the polymerase chain reaction (PCR) to quantitatively amplify species-specific mRNAs that encode enzymes that regulate metabolic flux. In order to design the necessary primers for PCR, the cDNAs that encode for the enzymes, hexokinase, phosphofructokinase, phosphoglucose isomerase and citrate synthetase will be cloned utilizing the phylogenetically conserved primary sequences of these enzymes. Species-specific primers will be designed from these cDNA sequences and used to quantitatively amplify each enzyme's mRNA only from C. finmarchicus to ascertain the affect of environmental parameters on the level of enzyme expression. Differences in the quantity of C. finmarchicus present in a taxonomically complex assemblage of zooplankton will be normalized by simultaneously measuring the specific concentration of a physiologically invariant gene, such as actin. The results will be equivalent to enzyme per weight of C. finmarchicus, a unit comparable to more traditional physiological studies.
该项目为识别、分类和评估浮游动物的生理状态提供了一个独特的解决方案。分子技术将被开发,可以快速识别和量化在桡足动物,Calanus finmarchicus生理上重要的基因表达水平。这些技术利用聚合酶链反应(PCR)的特异性和敏感性,定量扩增编码调节代谢通量的酶的物种特异性mrna。为了设计PCR所需的引物,将利用这些酶的系统发育保守的一级序列克隆这些酶的编码cdna,包括己糖激酶、磷酸果糖激酶、磷酸葡萄糖异构酶和柠檬酸合成酶。根据这些cDNA序列设计物种特异性引物,定量扩增各酶mRNA,以确定环境参数对酶表达水平的影响。通过同时测量一个生理上不变的基因(如肌动蛋白)的特定浓度,可以使在分类上复杂的浮游动物组合中存在的C. finmarchicus数量的差异归一化。结果将相当于C. finmarchicus每重量的酶,一个可与更传统的生理研究相媲美的单位。

项目成果

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Lewis Incze其他文献

Lewis Incze的其他文献

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{{ truncateString('Lewis Incze', 18)}}的其他基金

CNH: Collaborative Research: Direct and Indirect Coupling of Fisheries Through Economic, Regulatory, Environmental, and Ecological Linkages
CNH:合作研究:通过经济、监管、环境和生态联系实现渔业的直接和间接耦合
  • 批准号:
    0709536
  • 财政年份:
    2007
  • 资助金额:
    $ 9.25万
  • 项目类别:
    Standard Grant
Collaborative Research: GLOBEC-01: Tidal Front Mixing and Exchange on Georges Bank: Controls on the Production of Phytoplankton, Zooplankton, and Larval Fishes
合作研究:GLOBEC-01:乔治滩潮汐锋混合和交换:对浮游植物、浮游动物和幼鱼生产的控制
  • 批准号:
    0228778
  • 财政年份:
    2002
  • 资助金额:
    $ 9.25万
  • 项目类别:
    Standard Grant
Collaborative Research: GLOBEC-01: Tidal Front Mixing and Exchange on Georges Bank: Controls on the Production of Phytoplankton, Zooplankton, and Larval Fishes
合作研究:GLOBEC-01:乔治滩潮汐锋混合和交换:对浮游植物、浮游动物和幼鱼生产的控制
  • 批准号:
    0322233
  • 财政年份:
    2002
  • 资助金额:
    $ 9.25万
  • 项目类别:
    Standard Grant
U.S. GLOBEC Phaes III: Environmental Consequences of Tidal- Front Entrainment in Larval Fish along the Southern Flank of Georges Bank
美国 GLOBEC Phaes III:潮汐锋面夹带乔治滩南侧幼鱼的环境后果
  • 批准号:
    9813642
  • 财政年份:
    1999
  • 资助金额:
    $ 9.25万
  • 项目类别:
    Standard Grant
U.S. GLOBEC: Dispersive and Advective Influences on the Survival of Cod and Haddock Larvae on Georges Bank
美国 GLOBEC:分散性和平流性对乔治浅滩鳕鱼和黑线鳕幼虫生存的影响
  • 批准号:
    9634165
  • 财政年份:
    1996
  • 资助金额:
    $ 9.25万
  • 项目类别:
    Continuing Grant
U.S.-GLOBEC: Vertical Distribution of Small Zooplankton During Turbulance Profiling on Georges Bank
U.S.-GLOBEC:乔治滩湍流剖面中小型浮游动物的垂直分布
  • 批准号:
    9313669
  • 财政年份:
    1995
  • 资助金额:
    $ 9.25万
  • 项目类别:
    Continuing Grant
Pelagic to Benthic Coupling in Lobster (Homarus americanus) Recruitment
龙虾(美洲白鹤)招募中的中上层与底栖耦合
  • 批准号:
    9202760
  • 财政年份:
    1992
  • 资助金额:
    $ 9.25万
  • 项目类别:
    Standard Grant

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