PCR ANALYSIS OF STARVATION INDUCED DNA REARRANGEMENTS

饥饿诱导的 DNA 重排的 PCR 分析

• 批准号: 9122654
• 负责人: James Shapiro
• 金额: $ 4.75万
• 依托单位: University of Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-01-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9122654&HistoricalAwards=false
• 关键词: PCR; ANALYSIS; STARVATION; INDUCED; DNA

It is now widely accepted that stress conditions lead to an increase in the frequencies of mutation measured by selection. The question now raised by the "Directed mutation" issue is the specificity of the changes induced by particular stress regimes. To answer this question, it is necessary to characterize all DNA changes regardless of whether they facilitate growth under selective conditions. This can now be done by PCR technology. PCR makes it possible to examine specific regions of the genome following stress and detect novel DNA structures, even if they can not be selected by growth. Initial results demonstrated that a specific class of DNA rearrangement mediated by the Mu phage transposable element did not measurably occur under normal growth conditions but were easily detectable at relatively high frequencies under selective conditions. Use of PCR will now allow detection of the frequency and time of occurance of such genetic rearrangements with and without the selective techniques that are usually required for measuring mutation rates.

现在人们普遍认为，压力条件会导致 通过选择测量的突变频率的增加。 的 “定向突变”问题现在提出的问题是， 由特定的压力制度引起的变化的特异性。到 为了回答这个问题，有必要描述所有的DNA 无论这些变化是否促进了增长， 选择性条件。 现在可以通过PCR技术来实现。 PCR 使得研究基因组的特定区域成为可能 并检测新的DNA结构，即使他们可以 不要选择成长。初步结果表明，A 由Mu噬菌体介导的特定类型的DNA重排 转座因子在正常生长条件下不存在 条件下，但在相对较高的温度下很容易检测到 频率选择条件下。 PCR的使用将允许 检测这种遗传性疾病发生的频率和时间 有和没有选择性技术的重排， 通常用于测量突变率。