Undergraduate Laboratory in Engineering Biology at Princeton University
Undergraduate Laboratory in Engineering Biology at Princeton University
批准号:
9152052
负责人:
Robert Prud'homme
金额:
$6.72万
依托单位:
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-09-01 至 1994-02-28
中文摘要
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英文摘要
A coherent set of laboratory experiments is developed that demonstrates the application of chemical engineering principles in biotechnology. This program is used in the undergraduate Chemical Engineering laboratory course to provide a core set of experiments that introduces Chemical Engineering students to biotechnology, and provides in-depth exposure for students in the newly instituted Engineering Biology Certificate Program. The laboratory experiments center around the production, isolation and purification of alkaline phosphatases from bacterial fermentations using either E. coli or Bacillus subtilis. The experiments follow the processes that would be used in the commercial production of the enzyme. They include: 1) a fermentation experiment, demonstrating microbial growth and product formation as well as O2 mass transfer; 2) an extraction experiment, demonstrating cell lysis and membrane permeabilization; 3) a cell harvesting experiment, comparing ultrafiltration and centrifugation and emphasizing scale-up considerations; 4) a purification experiment, demonstrating chromatographic and electrophoretic methods for isolation and characterization of proteins; and 5) an enzyme kinetics experiment, demonstrating methods for testing enzyme mechanisms by analysis of the kinetics of product inhibition.
期刊论文(0)
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科研奖励(0)
会议论文
Collaborative Research: SuSChEM: Root-Targeted Delivery of Encapsulated Agrochemicals using Natural Microbial Carriers
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批准号:1605816
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项目类别:Standard Grant
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资助金额:$11.11万
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财政年份:2016
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负责人:Robert Prud'homme
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依托单位:
NIRT: Functional Nanoparticle Formation by Block Copolymer Directed Assembly
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批准号:0506966
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项目类别:Standard Grant
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资助金额:$135.0万
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财政年份:2005
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负责人:Robert Prud'homme
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依托单位:
Hyperthermophilic Enzyme Modification of Polysaccharide Biopolymers: Interrelationship Between Biocatalysis and Rheology
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批准号:0121794
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项目类别:Continuing Grant
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资助金额:$28.92万
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财政年份:2001
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负责人:Robert Prud'homme
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依托单位:
Thermophilic Enzyme Hydrolysis of Water-Soluble Polymers: Interrelationship Between Biocatalysis and Rheology
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批准号:9711781
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项目类别:Standard Grant
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资助金额:$17.57万
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财政年份:1997
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负责人:Robert Prud'homme
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依托单位:
Presidential Young Investigator Award: Rheological Properties of Structured Fluids
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批准号:8351104
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项目类别:Continuing Grant
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资助金额:$18.75万
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财政年份:1984
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负责人:Robert Prud'homme
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依托单位:
Industry/University Cooperative Research Activity: Fundamentals and Applications of Hydrodynamic Chromatographic Separations
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批准号:8003320
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项目类别:Continuing Grant
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资助金额:$18.83万
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财政年份:1980
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负责人:Robert Prud'homme
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依托单位:
Research Initiation - Interactions of Polymer and SurfactantMolecules in Aqueous Solutions
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批准号:7908165
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项目类别:Standard Grant
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资助金额:$3.2万
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财政年份:1979
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负责人:Robert Prud'homme
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依托单位:
海外基金