Undergraduate Laboratory in Engineering Biology at Princeton University

开发了一套连贯的实验室实验，展示了化学工程原理在生物技术中的应用。本课程用于化学工程本科实验室课程，提供一套核心实验，向化学工程学生介绍生物技术，并为新设立的工程生物学证书课程的学生提供深入的接触。实验室实验的中心是利用大肠杆菌或枯草芽孢杆菌从细菌发酵中生产、分离和提纯碱性磷酸酶。这些实验遵循了将用于该酶的商业生产的过程。它们包括：1)发酵实验，演示微生物的生长和产物的形成以及O2的传质；2)提取实验，演示细胞裂解和膜渗透；3)细胞收获实验，比较超滤和离心法，并强调放大考虑；4)纯化实验，展示了分离和鉴定蛋白质的层析和电泳法；5)酶动力学实验，通过对产物抑制动力学的分析，展示了测试酶机制的方法。

A coherent set of laboratory experiments is developed that demonstrates the application of chemical engineering principles in biotechnology. This program is used in the undergraduate Chemical Engineering laboratory course to provide a core set of experiments that introduces Chemical Engineering students to biotechnology, and provides in-depth exposure for students in the newly instituted Engineering Biology Certificate Program. The laboratory experiments center around the production, isolation and purification of alkaline phosphatases from bacterial fermentations using either E. coli or Bacillus subtilis. The experiments follow the processes that would be used in the commercial production of the enzyme. They include: 1) a fermentation experiment, demonstrating microbial growth and product formation as well as O2 mass transfer; 2) an extraction experiment, demonstrating cell lysis and membrane permeabilization; 3) a cell harvesting experiment, comparing ultrafiltration and centrifugation and emphasizing scale-up considerations; 4) a purification experiment, demonstrating chromatographic and electrophoretic methods for isolation and characterization of proteins; and 5) an enzyme kinetics experiment, demonstrating methods for testing enzyme mechanisms by analysis of the kinetics of product inhibition.