Hyperthermophilic Enzyme Modification of Polysaccharide Biopolymers: Interrelationship Between Biocatalysis and Rheology

多糖生物聚合物的超高温酶修饰：生物催化与流变学之间的相互关系

• 批准号: 0121794
• 负责人: Robert Prud'homme
• 金额: $ 28.92万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-15 至 2005-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0121794&HistoricalAwards=false
• 关键词: Hyperthermophilic; Enzyme; Modification; Polysaccharide; Biopolymers

This proposal builds on an ongoing interdisciplinary effort to examine the interrelationship between enzymatic modification of water-soluble polymers and the resulting rheological consequences. The expanded scope of this project has the following objectives: (1) biochemical and biophysical characterization of promising wild-type and mutated hyperthermophilic galactomannanases and galactosidases, with respect to polysaccharide modification; (2) investigation of the mobility and distribution of enzymes within the biopolymer matrix during enzymatic modification along with the concomitant changes in the aggregation pattern of the biopolymer-enzyme system; and (3) correlation of enzyme activity to changes in rheology and galactomannan molecular architecture. Such an effort, based on the collective expertise of this research team, i.e., polysaccharide systems, enzymology at elevated temperatures, and the rheology of gels and solutions, will provide a comprehensive understanding of the fundamental and technological issues governing polymer modification resulting from enzyme action. While the focus here is on galactomannans, the long-term impact of this work will extend to strategic biocatalytic approaches to enhance the efficacy and use of other carbohydrate biopolymers.

这项建议建立在一个正在进行的跨学科的努力，以检查酶改性的水溶性聚合物和由此产生的流变学后果之间的相互关系。该项目的扩展范围具有以下目标：（1）关于多糖修饰，有希望的野生型和突变的超嗜热半乳甘露聚糖酶和半乳甘露聚糖酶的生物化学和生物物理特性;（2）在酶修饰过程中，随着生物聚合物-酶系统聚集模式的伴随变化，沿着，研究酶在生物聚合物基质中的移动性和分布;以及（3）酶活性与流变学和半乳甘露聚糖分子结构变化的相关性。 这样的努力，基于这个研究小组的集体专业知识，即，多糖系统，高温下的酶学，以及凝胶和溶液的流变学，将提供对酶作用引起的聚合物改性的基本和技术问题的全面理解。 虽然这里的重点是半乳甘露聚糖，但这项工作的长期影响将扩展到战略性生物催化方法，以提高其他碳水化合物生物聚合物的功效和使用。