Thermophilic Enzyme Hydrolysis of Water-Soluble Polymers: Interrelationship Between Biocatalysis and Rheology

水溶性聚合物的嗜热酶水解：生物催化与流变学之间的相互关系

• 批准号: 9711781
• 负责人: Robert Prud'homme
• 金额: $ 17.57万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 2000-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9711781&HistoricalAwards=false
• 关键词: Thermophilic; Enzyme; Hydrolysis; Water; Soluble

9711781 Prud'homme Aqueous solutions of galactomannan-based natural polymers are used extensively as hydraulic fracturing fluids to enhance productivity from gas and oil wells. Chemical or enzymatic hydrolysis of the galactomannan polymers is necessary to remove the polymer residue and open the fracture for oil or gas flow. For environmental and processing reasons, the use of hemicellulosic enzymes has become the preferred route for hydrolysis. However, little is known about the mechanisms by which these enzymes hydrolyze galactomannans or about the relationship among biocatalysis, physical chemistry, and rheology of these systems. The objectives of this collaborative study are to understand the physical chemistry and rheology of existing and proposed polymeric fracturing solutions during enzymatic hydrolysis at high temperatures. Results are to produce a new generation of stimulation fluids that would increase accessible oil and gas reserves, demonstrate the technological utility of a new class or termostable and thermoactive enzymes, and provide a fundamental understanding of the kinetics and mechanism of hydrolysis of galactomannan solutions and gels. ***

9711781 Prud'homme 基于半乳甘露聚糖的天然聚合物的水溶液广泛用作水力压裂液，以提高气井和油井的生产力。 半乳甘露聚糖聚合物的化学或酶水解对于去除聚合物残留物并打开裂缝以供石油或天然气流动是必要的。 出于环境和加工原因，使用半纤维素酶已成为水解的首选途径。 然而，人们对这些酶水解半乳甘露聚糖的机制或这些系统的生物催化、物理化学和流变学之间的关系知之甚少。 这项合作研究的目的是了解现有和拟议的聚合物压裂解决方案在高温酶水解过程中的物理化学和流变学。 其结果是产生新一代的增产液，从而增加可利用的石油和天然气储量，展示新型热稳定酶和热活性酶的技术实用性，并提供对半乳甘露聚糖溶液和凝胶水解动力学和机制的基本了解。 ***