Detection of Human Pathogenic Viruses in Aquatic Environments Using the Polymerase Chain Reaction

利用聚合酶链式反应检测水生环境中的人类致病病毒

• 批准号: 9201324
• 负责人: Donald Harleman
• 金额: --
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-15 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9201324&HistoricalAwards=false
• 关键词: Detection; Human; Pathogenic; Viruses; Aquatic

This is an award to support research on a technique to determine the presence, identity if present and numbers of pathogenic organisms, if present, in environmental waters. The technique is based on determination of the nature of the genetic material that is unique to the different target pathogens using an analytical procedure that amplifies the nucleic acid chain of the target organisms for ease of identification and quantification. The research involves concentration of the pathogens using a rotating membrane ultrafiltration medium and subsequent detection of the pathogens using the polymerase chain reaction procedure. This research addresses limitations of procedures currently used for determining the microbiological quality of an environmental water. These procedures depend on the analysis for surrogate microorganisms as a step toward establishing the significance of their presence. This project is a renewal of work initiated under NSF Small Grant for Exploratory Research No. 92-01324 results of which were utilized to develop the research plan for this project.

这是一个支持技术研究的奖项， 确定是否存在、身份（如果存在）和数量 环境沃茨中的病原生物（如果存在）。 该技术是基于确定的性质， 不同物种所特有的遗传物质 使用分析方法靶向病原体， 扩增目标生物体的核酸链 以便于识别和量化。 的 研究涉及使用一种 旋转膜超滤介质及后续 使用聚合酶链检测病原体 反应程序 这项研究解决了程序的局限性 目前用于测定微生物质量 一种环境水。 这些过程取决于 分析替代微生物， 确立他们存在的意义。 这 该项目是在NSF Small下启动的工作的更新 探索性研究补助金第92-01324号 这些数据被用来制定研究计划， 项目