Detection of Pathogenic Viruses in the Water Environment Using DNA Amplification Techniques

利用 DNA 扩增技术检测水环境中的致病病毒

• 批准号: 9014979
• 负责人: Donald Harleman
• 金额: --
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9014979&HistoricalAwards=false
• 关键词: Detection; Pathogenic; Viruses; Water; Environment

This is an award to support exploratory research on a technique to identify and quantify viral content of environmental waters. The technique is based on artificial synthesis of nucleic acid sequences that will be used as primers for a polymerase chain reaction that amplifies the nucleic acid chain of the target virus for identification and quantification. This proposal was submitted to NSF under conditions outlined in NSF 89-85, Small Grants for Exploratory Research (SGER). The proposed research addresses limitations of current approaches for determining the quality of a water supply by use of surrogate parameters rather than by search and analysis for the target itself.

这是一个奖项，以支持一种技术的探索性研究，以确定和量化环境水的病毒含量。该技术基于人工合成核酸序列，这些序列将用作聚合酶链反应的引物，该反应可扩增目标病毒的核酸链，以进行鉴定和定量。该提案在NSF 89-85探索性研究小额赠款（SGER）中概述的条件下提交给NSF。拟议的研究解决了目前通过使用替代参数而不是通过对目标本身进行搜索和分析来确定供水质量的方法的局限性。