Detection of Pathogenic Viruses in the Water Environment Using DNA Amplification Techniques

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This is an award to support exploratory research on a technique to identify and quantify viral content of environmental waters. The technique is based on artificial synthesis of nucleic acid sequences that will be used as primers for a polymerase chain reaction that amplifies the nucleic acid chain of the target virus for identification and quantification. This proposal was submitted to NSF under conditions outlined in NSF 89-85, Small Grants for Exploratory Research (SGER). The proposed research addresses limitations of current approaches for determining the quality of a water supply by use of surrogate parameters rather than by search and analysis for the target itself.