RUI: Variation in the Dispersal of Fertilizing Sperm as a Function of Population Density

RUI：受精精子传播的变化作为人口密度的函数

• 批准号: 9202805
• 负责人: Philip Yund
• 金额: $ 9.36万
• 依托单位: University of New Orleans
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1994-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9202805&HistoricalAwards=false
• 关键词: RUI; Variation; Dispersal; Fertilizing; Sperm

I seek funding to explore the impact of variation in population density on the dispersal distance of fertilizing sperm (those sperm that successfully fertilize an egg, as opposed to all of the sperm that are released) and the incidence of multiple paternity in a colonial ascidian. Work to date has demonstrated that: 1) Sperm concentration in the field decreases rapidly with distance from isolated males. 2) The fertilization rate of eggs is a function of sperm concentration. These preliminary data suggest that the dispersal distance of fertilizing sperm when multiple males are contributing to fertilization will be much shorter than the distances previously reported for isolated males. If the probability of a given male fertilizing an egg is proportional to the concentration of his sperm relative to that of other males, then the dispersal distance of fertilizing sperm will decrease as population density increases. As a consequence, more males may participate in the fertilization of each female at higher population densities, leading to an increase in multiple paternity within a brood of larvae. I propose to test these hypotheses by establishing populations of ascidians with known genetic markers at different densities and conducting paternity analyses to assay the paternity of brooded larvae after fertilization has occurred. This study will contribute to our knowledge of benthic marine organisms by examining the potential for variation in population density to impact aspects of population structure such as interbreeding population size, levels of inbreeding, relatedness of neighboring individuals or colonies, and gene flow among sub- populations.

我寻求资金来探索人口变化的影响 密度对精子扩散距离的影响（ 精子成功地使卵子受精，而不是所有 精子被释放）和多个 殖民地海鞘的父权。 迄今为止的工作表明， 即： 1)现场的精子浓度迅速下降， 远离孤立的男性。 2)卵子的受精率是精子的函数 浓度. 这些初步数据表明， 当多个雄性参与时， 受精的距离将比以前短得多 报告了孤立的男性。 如果一个雄性 鸡蛋的浓度与他的 精子相对于其他男性，然后分散 随着种群密度的增加，精子间的距离逐渐减小 增大 因此，更多的男性可能会参加 在较高的种群密度下， 导致了一窝企鹅中多父性的增加， 幼虫 我建议通过建立一个 在不同温度下具有已知遗传标记的海鞘种群 密度和进行亲子分析，以测定 受精后孵卵幼虫的亲子关系已经发生。 本研究将有助于加深对海洋底栖生物的认识 通过检查种群变异的可能性， 密度影响人口结构的各个方面， 杂交种群规模，近交水平，亲缘关系 邻近个体或群体的基因流，以及亚群体之间的基因流， 人口。