Regulation of Pyrimidine Biosynthesis and Cell Growth

嘧啶生物合成和细胞生长的调节

• 批准号: 9205091
• 负责人: Stuart Tsubota
• 金额: $ 23.64万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9205091&HistoricalAwards=false
• 关键词: Regulation; Pyrimidine; Biosynthesis; Cell; Growth

The regulatory mechanisms utilized for the control of expression of the rudimentary gene of Drosophila melanogaster will be examined with a combination of genetic and molecular approaches. The underlying hypothesis is that there exist a number of trans- acting regulatory genes which interact with the rudimentary gene to produce the developmental program of gene expression. The tissue- specific control regions within the 5'end of the rudimentary gene will be more precisely mapped and characterized. Fusion proteins will be isolated and used to generate antibodies to the wild-type mod(r)1 and mod(r)2 proteins. These antibodies will be used to determine the tissue distribution and the cellular localization of the wild-type proteins. Additionally, the antibodies will be used for the characterization and purification of the gene products. Lastly, the null phenotypes and normal functions of mod(r)1 and mod(r)2 will be determined. Mutants which have deficiencies and have a null phenotype will be isolated and characterized. cDNA's will continue to be isolated and sequenced in an effort to determine the biological function of these genes. %%% An understanding of the many ways in which genes are regulated so that the precise developmental pattern of a living organism almost invariably occurs is critical to our insight into life processes.

用于控制 果蝇发育基因的表达， 用遗传学和分子学方法相结合的方法进行检查。 基本假设是，存在一些跨- 作用调节基因与基本基因相互作用， 产生基因表达的发育程序。组织- 在基本基因的5 '端内的特定控制区域 将被更精确地描绘和描述。融合蛋白 将被分离并用于产生针对野生型的抗体 mod（r）1和mod（r）2蛋白。这些抗体将用于 确定组织分布和细胞定位 野生型蛋白质。此外，还将使用抗体 用于基因产物的表征和纯化。 最后，对mod（r）1和mod（r）2的空表型和正常函数进行了研究。 mod（r）2将被确定。有缺陷的突变体， 将被分离和表征。cDNA序列 将继续被分离和排序， 决定这些基因的生物学功能。 %%% 了解基因调控的多种方式， 生物体的精确发育模式 对我们了解生命过程至关重要。