Genetic and Molecular Analysis of Sucrose Metabolism in Maize
玉米蔗糖代谢的遗传和分子分析
基本信息
- 批准号:9205213
- 负责人:
- 金额:$ 25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1992
- 资助国家:美国
- 起止时间:1992-07-15 至 1996-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Import and metabolism of sucrose is essential to growth and development of non-photosynthetic cells in the vast majority of plant species. Only two enzymatic reactions can catalyze sucrose breakdown; sucrose synthase or invertase. The physiological significance of each pathway has been widely debated. Recent advances in molecular analysis of the genes encoding these enzymes have opened up exciting possibilities for resolving these issues. We have previously shown that the sucrose synthase genes of maize are differentially regulated by sugar availability. Recently we have been able to extend this combined molecular genetic and physiological approach to the invertase genes in maize. A cDNA clone for maize invertase and identified maize lines with genetic deficiencies in invertase expression has been isolated. In particular, we have focused a maize genotype that specifically lacks invertase activity in the primary root system, leaving the whole plant free from direct effects of the deficiency. These proposed experiments will: 1) Characterize a pair of maize invertase genes - their structure, expression, and physilogical function, 2) Determine the genetic basis and physiological consequences of a primary root invertase deficiency in maize, 3) Test the effects of double and triple mutant combinations of sucrose synthase and invertase deficiencies.
蔗糖的输入和代谢是绝大多数植物非光合细胞生长发育所必需的。只有两种酶促反应能催化蔗糖分解;蔗糖合酶或蔗糖转化酶。每条通路的生理意义一直备受争议。编码这些酶的基因的分子分析的最新进展为解决这些问题开辟了令人兴奋的可能性。我们以前已经表明,玉米的蔗糖合酶基因受到糖可用性的不同调节。最近,我们已经能够将这种结合分子遗传学和生理学的方法扩展到玉米的转化酶基因。已分离出一个玉米转化酶cDNA克隆和已鉴定的转化酶表达遗传缺陷玉米系。我们特别关注了一种玉米基因型,该基因型在主根系统中缺乏转化酶活性,使整个植株免受缺乏转化酶的直接影响。这些拟议的实验将:1)表征一对玉米转化酶基因-它们的结构,表达和生理功能;2)确定玉米主根转化酶缺乏的遗传基础和生理后果;3)测试蔗糖合酶和转化酶缺乏的双重和三重突变组合的影响。
项目成果
期刊论文数量(0)
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Karen Koch其他文献
Antecedents Influencing Rugby Migration In South Africa
影响南非橄榄球移民的前因
- DOI:
10.19030/iber.v11i11.7371 - 发表时间:
2012 - 期刊:
- 影响因子:0
- 作者:
S. Pienaar;Karen Koch - 通讯作者:
Karen Koch
Over-expression of emXA21 binding protein 3/em enhances rice survival under water-deficit stress
水稻XA21结合蛋白3的过表达提高了水稻在缺水胁迫下的存活率
- DOI:
10.1016/j.plantsci.2025.112454 - 发表时间:
2025-05-01 - 期刊:
- 影响因子:4.100
- 作者:
Xiaoen Huang;Xiuhua Chen;Satyam Vergish;Xiaodong Ding;Xiaofei Liang;Sixue Chen;Karen Koch;Wen-Yuan Song - 通讯作者:
Wen-Yuan Song
Karen Koch的其他文献
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{{ truncateString('Karen Koch', 18)}}的其他基金
EAGER: An Unexplored Avenue of Striga Resistance in Maize
EAGER:玉米中尚未探索的Striga抗性途径
- 批准号:
1521100 - 财政年份:2015
- 资助金额:
$ 25万 - 项目类别:
Standard Grant
Functional Analysis of Maize Genes for Sucrose Metabolism
玉米蔗糖代谢基因的功能分析
- 批准号:
0080282 - 财政年份:2000
- 资助金额:
$ 25万 - 项目类别:
Continuing grant
Sugar-Modulated Expression and Developmental Control of Genes for Sucrose Metabolism
蔗糖代谢基因的糖调节表达和发育控制
- 批准号:
9507681 - 财政年份:1995
- 资助金额:
$ 25万 - 项目类别:
Continuing grant
Physiological Role of Sugar-Modulated Gene Expression in Higher Plants
糖调节基因表达在高等植物中的生理作用
- 批准号:
8816776 - 财政年份:1989
- 资助金额:
$ 25万 - 项目类别:
Continuing Grant
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