Biochemistry of Fertilization and Early Development

PI提出，海胆受精时钙升高的主要结果是钙调蛋白依赖酶NAD激酶的激活，该酶产生NADP。钙的升高也激活了葡萄糖-6-磷酸脱氢酶，导致NADP迅速还原为NADPH。随之而来的NADPH/NADP比率的增加使细胞的氧化还原平衡转向有利于还原，这对蛋白质合成激活有影响。其他监管方面的变化源于钙价的上涨。这项研究的具体目标是：1)肯定或否定NADPH改变在调节体内蛋白质合成中的作用；2)确定NADPH/NADP比率的改变是否还通过蛋白质二硫基的改变起作用，如通过硫氧还蛋白或其他蛋白质氧化还原酶系统。

The PI proposes that a major consequence of the calcium rise at fertilization in the sea urchin isthe activation of the calmodulin dependend enzyme, NAD kinase, which produces NADP. The calcium rise also activates Glucose-6-phosphate dehydrogenase, with the result that the NADP is rapidly reduced to NADPH. The resultant increase in the NADPH/NADP ratio then shifts the redox poise of the cell to favor reduction which has consequences for protein synthesis activation. Other regulatory changes emanate from the calcuim rise. The specific goals of the proposed research are to 1) affirm or negate the role of the NADPH change in regulating protein synthesis in vivo and 2) determine whether the NADPH/NADP ratio change is, in addition, acting through alteration of protein disulphide groups, as through a thioredoxin or other protein oxidoreductase system.