Biochemistry of Fertilization and Early Development

PI 提出，海胆受精时钙含量升高的一个主要后果是钙调蛋白依赖性酶 NAD 激酶的激活，该酶产生 NADP。钙的升高还会激活 6-磷酸葡萄糖脱氢酶，从而使 NADP 迅速还原为 NADPH。由此产生的 NADPH/NADP 比率的增加然后改变细胞的氧化还原平衡以利于还原，这对蛋白质合成激活产生影响。其他监管变化源于钙的增长。拟议研究的具体目标是 1) 肯定或否定 NADPH 变化在调节体内蛋白质合成中的作用，2) 确定 NADPH/NADP 比率变化是否另外通过改变蛋白质二硫基团（如通过硫氧还蛋白或其他蛋白质氧化还原酶系统）发挥作用。

The PI proposes that a major consequence of the calcium rise at fertilization in the sea urchin isthe activation of the calmodulin dependend enzyme, NAD kinase, which produces NADP. The calcium rise also activates Glucose-6-phosphate dehydrogenase, with the result that the NADP is rapidly reduced to NADPH. The resultant increase in the NADPH/NADP ratio then shifts the redox poise of the cell to favor reduction which has consequences for protein synthesis activation. Other regulatory changes emanate from the calcuim rise. The specific goals of the proposed research are to 1) affirm or negate the role of the NADPH change in regulating protein synthesis in vivo and 2) determine whether the NADPH/NADP ratio change is, in addition, acting through alteration of protein disulphide groups, as through a thioredoxin or other protein oxidoreductase system.