Molecular Bases of Antioxidant Responses in Arabidopsis

拟南芥抗氧化反应的分子基础

• 批准号: 9205515
• 负责人: Ruth Grene
• 金额: $ 3.83万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-11-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9205515&HistoricalAwards=false
• 关键词: Molecular; Bases; Antioxidant; Responses; Arabidopsis

Ruth Alscher, of the Department of Plant Pathology, Physiology and Weed Science at Virginia Polytechnic and State University is applying to spend a year at the DOE/MSU Plant Research Laboratory on the Michigan State University campus in the laboratory of Dr. Chris Somerville. The title of her research project will be Molecular bases of antioxidant responses in Arabidopsis. The mechanisms in green leaf cells which protect against oxidative stress will be studied in Arabidopsis, in particular the production of three plastid proteins specifically associated with resistance whose inheritance may be coordinately controlled. These antioxidant defense proteins have been implicated in resistance to a number of different stresses, including drought, extremes of temperature, herbicides and air pollutants. The PI will avail herself of the expertise and resources of Dr. Sommerville's laboratory to study effects of exposure to stress on gene expression, to identify mutants with a altered sensitivity to oxidative stress and to study the signalling process by which altered gene expressioning is brought about. One principal focus will be the development of a molecular cloning strategy to identify the genes which encode the antioxidant plastid proteins. A year of concentration in the molecular aspects of stress responses in green plant cells will enable the PI to progress towards her goal of understanding the basis in molecular genetics of differential resistance to oxidative stress in photosynthetic tissue.

Ruth Alscher，植物病理学，生理学和 弗吉尼亚理工大学和州立大学的杂草科学是 申请在DOE/MSU植物研究实验室工作一年 在密歇根州立大学校园里， 克里斯萨默维尔。 她的研究项目的标题将是 拟南芥抗氧化反应的分子基础。 的 绿色叶细胞中的抗氧化机制 将在拟南芥中研究胁迫，特别是 三种与抗性相关的质体蛋白 其遗传可以被协调地控制。这些抗氧化 防御蛋白质与对许多细菌的抗性有关， 不同的压力，包括干旱，极端的温度， 除草剂和空气污染物。 PI将利用 Sommerville博士实验室的专业知识和资源 暴露于压力对基因表达的影响，以确定 对氧化应激敏感性改变的突变体， 改变基因表达的信号传导过程是 带来的。 一个主要的重点将是发展一个 分子克隆策略，以确定编码 抗氧化质体蛋白。 一年专注于 绿色植物细胞中胁迫反应的分子方面将 使PI能够朝着她理解 抗氧化性差异的分子遗传学基础 光合组织中的胁迫。