Genetic Control of Organ Development in a Simple Eukaryote

主 本项目的目标是阐明分子机制 Frt 1基因的克隆可以诱导蘑菇的发育 （子实体）在木腐菌裂褶菌公社。 从相应的蛋白质序列预测的蛋白质序列的分析 cDNA和其他数据表明，Ftr 1编码一种膜- 相关蛋白质通过某种形式的自我与 非自我认知，其活动受 交配型基因和连锁遗传元件的活性。 这些概念将通过以下方法进行测试：1）一个标签， 确定FRT 1在细胞内的位置。 2)采用 体内诱变技术开始确定哪些部分 Frt 1序列的突变对于活性是必需的。 3)分离和 开始描述连接的DNA序列，除了 Frt 1，对于结果是必不可少的，这将使用以下方法完成： 的DNA介导的转化，以测试Frt 1侧翼序列 因为它们能够恢复植物的结实表型， 合适的Frt 1缺失突变。 4)隔离并启动 通过使用Frt 1表征Frt 1的替代等位基因，和/或 侧翼序列，作为替代菌株克隆库的探针 色葡萄公社。 %%% 通过研究基因调控阐明的机制概念 这个重要的发展过程在这个相对简单的 真核生物应该适用于理解类似的 在更复杂、更难接近的生物体中进行。

The primary objective of this project is to elucidate the molecular mechanisms by which a cloned gene called Frt1 induces development of mushrooms (fruiting bodies) in the woodrotting fungus Schizophyllum commune. Analysis of the protein sequence predicted from the corresponding cDNA, and other data, indicate that Ftr1 encodes a membrane- associated protein that functions through some form of self versus non-self recognition and that its activity is influenced by activity of the mating-type genes and a linked genetic element. These concepts will be tested by the following methods:1) a tag to determine the location of FRT1 within cells. 2) Employ the techniques of in vivo mutagenesis to start determining which parts of the Frt1 sequence are essential for activity. 3) Isolate and start characterizing the linked DNA sequence which, in addition to Frt1, is essential for fruiting this will be done using the method of DNA-mediated transformation to test the sequences flanking Frt1 for their ability to restore the fruiting phenotype in the appropriate Frt1 deletion mutations. 4) Isolate and start characterizing an alternate allele of Frt1 by using Frt1 , and/or flanking sequences, as probe to a clone bank of an alternate strain of S. commune. %%% Mechanistic concepts elucidated by studying genetic regulation of this important developmental process in this relatively simple eukaryote should be applicable to an understanding of similar processes in more complex, less accessible organisms.