Genetic Control of Organ Development in a Simple Eukaryote

本项目的主要目的是阐明一个名为Frt1的克隆基因在木本真菌裂叶菌（Schizophyllum commune）中诱导蘑菇（子实体）发育的分子机制。对相应cDNA预测的蛋白质序列和其他数据的分析表明，Ftr1编码一种膜相关蛋白，该蛋白通过某种形式的自我识别和非自我识别发挥作用，其活性受到交配型基因和相关遗传元件活性的影响。这些概念将通过以下方法进行测试：1)一个标签来确定FRT1在细胞内的位置。2)利用体内诱变技术开始确定Frt1序列的哪些部分对活性至关重要。3)分离并开始表征除Frt1外对结果至关重要的连锁DNA序列，这将使用DNA介导转化的方法来测试Frt1两侧的序列在适当的Frt1缺失突变中恢复结果表型的能力。4)利用Frt1和/或侧翼序列作为探针，分离出Frt1的一个交替等位基因，并开始对其进行鉴定。通过研究这种相对简单的真核生物中这一重要发育过程的遗传调控所阐明的机制概念，应该适用于理解更复杂、更不易接近的生物中的类似过程。

The primary objective of this project is to elucidate the molecular mechanisms by which a cloned gene called Frt1 induces development of mushrooms (fruiting bodies) in the woodrotting fungus Schizophyllum commune. Analysis of the protein sequence predicted from the corresponding cDNA, and other data, indicate that Ftr1 encodes a membrane- associated protein that functions through some form of self versus non-self recognition and that its activity is influenced by activity of the mating-type genes and a linked genetic element. These concepts will be tested by the following methods:1) a tag to determine the location of FRT1 within cells. 2) Employ the techniques of in vivo mutagenesis to start determining which parts of the Frt1 sequence are essential for activity. 3) Isolate and start characterizing the linked DNA sequence which, in addition to Frt1, is essential for fruiting this will be done using the method of DNA-mediated transformation to test the sequences flanking Frt1 for their ability to restore the fruiting phenotype in the appropriate Frt1 deletion mutations. 4) Isolate and start characterizing an alternate allele of Frt1 by using Frt1 , and/or flanking sequences, as probe to a clone bank of an alternate strain of S. commune. %%% Mechanistic concepts elucidated by studying genetic regulation of this important developmental process in this relatively simple eukaryote should be applicable to an understanding of similar processes in more complex, less accessible organisms.