RUI: Structure and Catalytic Mechanism of Plant Carbonic Anhydrase

RUI：植物碳酸酐酶的结构和催化机制

• 批准号: 9205892
• 负责人: Roger Rowlett
• 金额: $ 11万
• 依托单位: Colgate University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9205892&HistoricalAwards=false
• 关键词: RUI; Structure; Catalytic; Mechanism; Plant

Dr. Rowlett will isolate and purify carbonic anhydrase, an enzyme thought to be important species such as corn. Once isolated in pure form, he will examine the structure of the enzyme and several laboratory-modified derivatives using UV-visible spectroscopy, and powerful X-ray absorption techniques. Dr. Rowlett also proposed to investigate the catalytic role of plant carbonic anhydrase in detail using nuclear magnetic resonance and stopped-flow spectroscopy methods which are capable of picking apart individual chemical steps in their mechanism of action. %%% Although carbonic anhydrases of animal origin are well-known, plant carbonic anhydrases are poorly understood, and what little is known indicates that the plant enzyme is evolutionarily distinct from carbonic anhydrases of animal origin. Thus, the characterization of plant carbonic anhydrases is highly significant, for two reasons. First, plant and animal carbonic anhydrase may represent one of a few pairs of enzymes in nature which have evolved from different origins to arrive at an identical function (convergent evolution). By comparing the structures of such convergently evolved enzymes, it will likely be possible to distinguish the essential and nonessential features of protein structure responsible for chemical catalysis. Second, the study of the catalytic ability of plant carbonic anhydrase may help resolve the exact function of plant carbonic anhydrase in photosynthesis, a function that is currently unknown, primarily because of a lack of fundamental information about the catalytic ability of the enzymes.

罗莱特博士将分离纯化碳酸酐酶， 被认为是玉米等重要物种。 一旦被隔离在 纯粹的形式，他将研究酶的结构和几个 使用紫外-可见光谱的实验室改性衍生物，和 强大的X射线吸收技术。 罗莱特博士还建议 探讨植物碳酸酐酶在植物中的催化作用， 详细使用核磁共振和停流 光谱学方法能够分辨出 在其作用机制中的化学步骤。 %%% 尽管动物来源的碳酸酐酶是众所周知的，但植物来源的碳酸酐酶是已知的。 对碳酸酐酶的了解很少， 表明植物酶在进化上与 动物来源的碳酸酐酶。 因此， 植物碳酸酐酶是非常重要的，两个 原因 首先，植物和动物的碳酸酐酶可能代表 是自然界中少数几对酶之一， 不同的起源达到相同的功能（收敛 进化）。 通过比较这种收敛的结构， 进化的酶，很可能可以区分 蛋白质结构的基本和非基本特征 负责化学催化。 第二，研究 植物碳酸酐酶的催化能力可能有助于解决 植物碳酸酐酶在光合作用中的确切作用， 目前还不清楚，主要是因为缺乏 关于酶的催化能力的基本信息。