Genetic Regulation of Streptomyces Antibiotics

链霉菌抗生素的基因调控

• 批准号: 9206068
• 负责人: Wendy Champness
• 金额: $ 30.7万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-15 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9206068&HistoricalAwards=false
• 关键词: Genetic; Regulation; Streptomyces; Antibiotics

Streptomyces are well-known for their capacity to synthesize a vast repertoire of secondary metabolites (antibiotics). Besides their commercial importance, the Streptomyces are interesting for their complex developmental cycle. In the course of this life cycle, antibiotic synthesis is developmentally regulated. The objective of this research is to define aspects of the global genetic regulation of Streptomcymes coelicolor antibiotic synthesis. The research planned is based on a set of genes, recently discovered in this laboratory, which regulate the expression of antibiotic genes. The goals include genetic and molecular characterization of the absA, absB and mia genes; study of the regulation of absA, absB and mia using reporter gene fusions, and investigation of the mechanism by which these genes globally regulate antibiotic synthesis. %%% This molecular genetic analysis of the abs and mia genes promises to contribute not only to our understanding of microbial developmental gene regulation, but also to biotechnology. Understanding of S. coelicolor regulation is highly likely to explain much of antibiotic regulation in other Streptomycetes and Actinomycetes, and this increase the potential for rational manipulation of production of commercially important metabolites including the many new metabolites that are continuously being discovered.

链霉菌以其合成大量 次级代谢产物（抗生素）的库。 除了他们 商业上的重要性，链霉菌是有趣的，因为它们 复杂的发展周期。 在这个生命周期中， 抗生素的合成是受发育调节的。 客观 这项研究的目的是确定全球遗传学的各个方面， 天蓝色链霉菌抗生素合成的调控。 的 计划中的研究是基于一组基因，最近发现， 这个实验室，调节抗生素基因的表达。 目标包括遗传和分子表征的 absA、absB和mia基因的调控研究 利用报告基因融合的mia及其机制的研究 这些基因通过它来全面调节抗生素的合成。 %%% 对abs和mia基因的分子遗传学分析表明， 不仅有助于我们理解微生物 发展基因调控，也是生物技术。 理解S。腔肠调控很可能 解释了其他链霉菌中的抗生素调节， 放线菌，这增加了合理的潜力， 商业上重要的代谢产物生产的操作 包括许多新的代谢物， 发现了