Molecular Genetics of Streptomyces Differentiation

链霉菌分化的分子遗传学

• 批准号: 8811338
• 负责人: Wendy Champness
• 金额: $ 15.4万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8811338&HistoricalAwards=false
• 关键词: Molecular; Genetics; Streptomyces; Differentiation

The objective of the grant is to define aspects of the genetic regulation of Streptomyces coelicolor morphological and physiological differentiation. The emphasis is on the transition from the vegetative to the differentiated colony, as manifested by onset of antibiotic synthesis and by the growth of prespore aerial hyphae. The specific aims are: 1. Genetic and phenotypic Characterization of the new developmental mutants. We will refine the genetic characterization of the newly identified absA, bldH loci by performing complementation and fine-structure mapping studies. We will also continue the phenotypic characterization of the bld mutants. 2. Cloning and molecular characterization of the new developmental genes absA, bldG and bldH. We will clone these genes and use the cloned alleles for complementation studies and for sequencing the wild-type and mutant alleles. This genetic and molecular analysis of the abs and bld genes promises to contribute not only to our understanding of microbial multicellular development and differentiation, but also to the potential for rational manipulation of naturally occurring antibiotic synthesis.

赠款的目的是确定遗传学的各个方面， 天蓝色链霉菌的形态调控 生理分化 重点是过渡 从营养体到分化群体， 通过抗生素合成的开始和前孢子的生长 气生菌丝 具体目标是： 1. 新基因的遗传和表型特征 发育突变体 我们将改进基因 新鉴定的absA、bldH基因座的表征 通过执行互补和精细结构映射 问题研究 我们还将继续表型 BLD突变体的表征。 2. 克隆和分子表征的新的 发育基因absA、bldG和bldH。 我们将克隆 这些基因，并使用克隆的等位基因 互补研究和测序野生型 和突变等位基因。 对abs和bld基因的遗传和分子分析 不仅有助于我们理解微生物 多细胞的发育和分化，而且对 合理利用自然资源的潜力 抗生素合成