Flavonoid-Induced Pollen Germination: Localization, Kinetics and Gene Isolation

类黄酮诱导花粉萌发：定位、动力学和基因分离

• 批准号: 9206712
• 负责人: Loverine Taylor
• 金额: --
• 依托单位: Washington State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9206712&HistoricalAwards=false
• 关键词: Flavonoid; Induced; Pollen; Germination; Localization

Most higher plants reproduce sexually, consequently factors that influence fertilization have a significant impact on reproductive success. Despite that little is known of the cellular and molecular events surrounding pollen germination, tube growth, and deposition of the sperm into the embryo sac. We established that flavonoids, normally found in pollen, are essential for successful fertilization. Flavonoid-deficient pollen is unable to germinate but this defect can be "rescued" by the exogenous application of micromolar quantities of a specific class of flavonoids leading to germination and tube growth in vitro and normal fertilization and seed-set in vivo. How do these small, hydrophobic molecules induce pollen germination? The basic tool to address this question if the pollen rescue assay. To begin, the accumulation site and intracellular transport mechanisms of flavonoids will be determined by localization and kinetic analysis. The identical flavonoid required for germination also stimulates transcription; this suggests a molecular mechanism of flavonoid action and requires that these genes be identified by cloning and sequencing. This will lead to a model of flavonoid action during pollen germination. The product of this proposal will extend the knowledge of basic reproductive biology and provide information useful in the design of mechanisms to manipulate plant fertility.

大多数高等植物有性繁殖，因此影响受精的因素对繁殖成功率有重要影响。尽管如此，人们对花粉萌发、管状生长和精子进入胚囊的细胞和分子过程知之甚少。我们确定，通常在花粉中发现的类黄酮是成功受精所必需的。缺乏黄酮类化合物的花粉无法发芽，但这种缺陷可以通过外源施加微摩尔量的特定类黄酮来“拯救”，从而在体外萌发和试管生长，在体内正常受精和结实子。这些小的疏水分子是如何诱导花粉萌发的？解决这个问题的基本工具是花粉拯救试验。首先，黄酮类化合物的积累位点和细胞内转运机制将通过定位和动力学分析确定。发芽所需的黄酮类化合物也刺激转录；这提示了类黄酮作用的分子机制，需要对这些基因进行克隆和测序鉴定。这将建立一个黄酮类化合物在花粉萌发过程中的作用模型。这一建议的成果将扩展基本生殖生物学的知识，并为设计操纵植物肥力的机制提供有用的信息。