RUI: Pattern Formation in the Heterophyllic Aquatic Angiosperm Hippuris Vulgaris: A Study of ABA-Inducible Stomatal and Vascular Development

叶片发育和图案形成的许多基本方面 对植物的了解很少。 这项研究将使用一种 研究水生植物两种特殊组织的发育 类型-气孔和侧脉（即维管组织）-在 叶子 该植物在本研究中具有独特的优势 因为这两种组织类型在沉水植物上通常不存在 叶，但它们可以被诱导分化的植物暴露 植物生长调节剂脱落酸（阿坝）。 因此， 系统提供了一个研究替代模式的机会， 在统一的遗传背景下发展。 拟议 研究将检验微管与 方向和ABA诱导的气孔发育 结合免疫荧光显微镜和微管- 扰乱毒品 将研究ABA诱导的血管发育 通过一系列的实验。 最后，一项基础研究 阿坝与细胞分裂速率的关系 在发育中的海马属植物叶子。 从这项研究中获得的结果 将增加对气孔和维管发育的理解 无论是在普通的海马中还是在一般的植物中。 Z^//

Many fundamental aspects of leaf development and pattern formation in plants are poorly understood. This research will use a species of aquatic plants to study the development of two specific tissue types - stomata and lateral veins (i.e. vascular tissue) - in leaves. This plant possesses unique advantages for this research because the two tissue types are normally absent on submerged plant leaves, but they can be induced to differentiate on plants exposed to the plant growth regulator abscisic acid (ABA). Thus, this system presents an opportunity to study alternative modes of development against a uniform genetic background. The proposed research will examine the relationship between microtubule orientation and ABA-induced stomatal development using a combination of immunofluorescence microscopy and microtubule- disrupting drugs. ABA-induced vascular development will be studied through a combination of experiments. Finally, a fundamental study will be a made of the relationship of ABA to cell division rates in developing Hippuris leaves. Results obtained from this research will increase understanding of stomatal and vascular development both in Hippuris vulgaris specifically and in plants in general. Z^//