Spatial Control of Pattern Formation in Early Vertebrate Development
早期脊椎动物发育中模式形成的空间控制
基本信息
- 批准号:10673415
- 负责人:
- 金额:$ 87.01万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-08-01 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
PROJECT SUMMARY / ABSTRACT
Embryonic development is defined by the emergence of spatial patterns of gene expression, which
organize progenitor cells into the blueprint of tissues and organs. To understand how genetic information
gives rise to tridimensional arrangements of cells, we need to assess how genomes are regulated in
space and time. While emerging technologies have allowed for precise quantification of gene expression
in single cells, strategies to integrate transcriptomic information and spatial cellular heterogeneity have
been lacking. Here, we propose to combine spatial transcriptomics and epigenomic profiling to
reconstruct the regulatory architecture of the early amniote embryo. By conducting RNA-seq in cryo-
sections of the avian embryo, we will establish a set of transcriptomic coordinates along the body axes.
These parameters will allow us to position single-cell RNA-seq datasets in a tridimensional grid that will
be used to reconstruct spatial patterns of gene expression on a genome-wide scale. To test this
approach we assembled a prototype of this virtual embryo, which is composed of pixels containing
regulatory information from all genes expressed in the genome. This model allowed us to recreate
spatial expression patterns, uncovering a high degree of regulatory complexity in the chick gastrula.
Since this is likely to be reflected in the genomic organization of gastrulating cells, a second aim of this
proposal is to use a Cartesian approach to explore chromatin regulation in space. We will establish how
chromatin accessibility and histone modifications change along the three axes of the embryo, and
assemble tridimensional maps of active genomic elements. We will use these high- resolution models of
the amniote gastrula to define the gene regulatory program that controls the emergence of distinct fields
of progenitor cells. Ultimately, our goal is to define how the gene regulatory networks operate in space
to drive pattern formation in the developing embryo.
项目摘要/摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Marcos Simoes-Costa其他文献
Marcos Simoes-Costa的其他文献
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{{ truncateString('Marcos Simoes-Costa', 18)}}的其他基金
Genomic control of neural crest identity by signaling systems
通过信号系统对神经嵴特性进行基因组控制
- 批准号:
10673423 - 财政年份:2022
- 资助金额:
$ 87.01万 - 项目类别:
Genomic control of neural crest identity by signaling systems
通过信号系统对神经嵴特性进行基因组控制
- 批准号:
10165694 - 财政年份:2019
- 资助金额:
$ 87.01万 - 项目类别:
Genomic control of neural crest identity by signaling systems
通过信号系统对神经嵴特性进行基因组控制
- 批准号:
10017179 - 财政年份:2019
- 资助金额:
$ 87.01万 - 项目类别:
Integrating signaling and transcriptional control in neural crest specification
将信号传导和转录控制整合到神经嵴规范中
- 批准号:
9379527 - 财政年份:2016
- 资助金额:
$ 87.01万 - 项目类别:
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