Lignin Degradation by Phanerochaete chrysophorium: Biochemical Characterization of Extracellular Peroxidases

黄原平革菌降解木质素：细胞外过氧化物酶的生化表征

• 批准号: 9207997
• 负责人: Michael Gold
• 金额: $ 30万
• 依托单位: Oregon Graduate Institute of Science & Technology
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-10-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9207997&HistoricalAwards=false
• 关键词: Lignin; Degradation; Phanerochaete; chrysophorium; Biochemical

The aim of the proposed project is to further our understanding of manganese peroxidase (MnP) and lignin peroxidase (LiP) from the lignin-degrading basidiomycete Phanerochaete chrysosporium. The underlying objectives of the proposed project are to further characterize the structure, mechanism and function of these enzymes and to clarify their role in lignin degradation. The binding, stoichiometry and mechanism of manganese oxidation by MnP will be studied by kinetic methods and by optical and NMR spectroscopy. Kinetic, spectroscopic and reactivity experiments also will be used to examine the mechanism of oxidation of syringyl and guaiacyl free phenolic dimeric model compounds by MnP and LiP from P. chrysosporium, and MnP and laccase form Dichomitus squalens. The mechanism of nonphenolic model compound degradation by D. squalens will also be examined. In addition, the mechanism of phenol oxidation by LiP and the mechanism of phenol inhibition of nonphenolic aromatic compound oxidation by LiP will be examined. Finally, using our P. chrysosporium DNA transformation system, a homologous expression system will be developed for these peroxidases. %%% Lignin is the second most abundant natural polymer in the biosphere and the most abundant renewable aromatic material. This research focusses on lignin degrading fungi and the structure and mechanisms of the enzymes responsible for catalyzing the degradation. The potential utilization of these micro organisms for transforming lignin into useful materials, chemicals and fuels is a very significant area of research.

建议项目的目的是加深我们对 锰过氧化物酶（MnP）和木质素过氧化物酶（LiP） 降解木质素的担子菌黄孢革革菌。的 拟议项目的基本目标是进一步 表征这些酶的结构、机制和功能 并阐明它们在木质素降解中的作用。 研究了锰氧化物的结合、化学计量和机理。 MnP将通过动力学方法以及光学和NMR进行研究 谱 动力学、光谱和反应性实验 并将其应用于研究丁香基的氧化机理 和愈创木基游离酚二聚体模型化合物通过MNP和LiP 来自P.chrysosporium，MNP和漆酶来自Dichomitus 角鲨非酚类模型化合物的降解机理 由D.并会检查角鲨烷。 此外，该机制 LiP对苯酚氧化的影响及其抑制机理 非酚类芳香化合物的氧化LiP将被检查。 最后，使用我们的P.chrysosporium DNA转化系统， 将开发这些同源表达系统 过氧化物酶。 %%% 木质素是生物圈中第二丰富的天然聚合物 和最丰富的可再生芳香材料。 本研究 重点介绍了木质素降解菌的结构和机理， 负责催化降解的酶。的 这些微生物用于转化的潜在用途 将木质素转化为有用的材料、化学品和燃料是一种非常重要的方法。 重要的研究领域。