Lignin Degradation by Phanerochaete Chrysosporium: Biochemical Characterization of Extracellular Peroxidases
黄孢原毛平革菌 (Phanerochaete Chrysosporium) 的木质素降解:细胞外过氧化物酶的生化表征
基本信息
- 批准号:8904358
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing grant
- 财政年份:1989
- 资助国家:美国
- 起止时间:1989-09-01 至 1993-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The overall objective of this project is to characterize the biochemical function of two enzymes, a lignin peroxidase (LiP) and a manganese peroxidase (MnP), in the oxidative degradation of lignin by the white-rot fungus Phanerochaete chrysosporium. Kinetic, spectroscopic and reactivity experiments will be conducted to ascertain the role of veratryl alcohol in the mechanism and regulation of LiP. Steady-state kinetic measurements, EPR, and resonance Raman spectroscopy will be used to characterize the active center of MnP and the role of Mn as redox couple in the enzyme. Lignin is the second most abundant biopolymer and the most abundant natural aromatic material on earth. An understanding of the role of these enzymes in the biodegradation of lignin may lead to the development of many useful biotechnological applications such as those in the pulp-paper and chemical industries. They would be less energy demanindg and more environmentally benign than existing processes.
该项目的总体目标是表征两种酶的生化功能,木质素过氧化物酶(LiP)和锰过氧化物酶(MNP),在氧化降解木质素的白腐真菌黄孢原毛革菌。动力学,光谱和反应性实验将进行,以确定藜芦醇的作用机制和调节的LiP。稳态动力学测量,EPR和共振拉曼光谱将被用来表征的活性中心的锰和锰作为氧化还原对的酶的作用。木质素是地球上第二丰富的生物聚合物和最丰富的天然芳香材料。了解这些酶在木质素生物降解中的作用可能会导致许多有用的生物技术应用的发展,如在制浆造纸和化学工业中的应用。与现有的工艺相比,它们对能源的需求更少,对环境更有利。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Michael Gold其他文献
Employment Policy in the European Union
欧盟的就业政策
- DOI:
- 发表时间:
2009 - 期刊:
- 影响因子:0
- 作者:
Michael Gold - 通讯作者:
Michael Gold
‘Taken on board’: An evaluation of the influence of employee board-level representatives on company decision-making across Europe
“采纳”:评估员工董事会级代表对整个欧洲公司决策的影响
- DOI:
10.1177/0959680110392276 - 发表时间:
2011 - 期刊:
- 影响因子:2.6
- 作者:
Michael Gold - 通讯作者:
Michael Gold
Corporate Social Responsibility and Trade Unions: Perspectives across Europe
企业社会责任与工会:整个欧洲的观点
- DOI:
- 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
Lutz Preuss;Michael Gold;C. Rees - 通讯作者:
C. Rees
Initial performance of red mulberry (Morus rubra L.) under a light gradient: an overlooked alternative livestock forage?
- DOI:
10.1007/s10457-021-00699-3 - 发表时间:
2021-10-27 - 期刊:
- 影响因子:2.200
- 作者:
Ryan Dibala;Shibu Jose;Michael Gold;Robert Kallenbach;Benjamin Knapp - 通讯作者:
Benjamin Knapp
Development of lipid nanoparticles for delivery of siRNA to neural cells
- DOI:
10.1016/j.jpain.2021.03.048 - 发表时间:
2021-05-01 - 期刊:
- 影响因子:
- 作者:
Purva Khare;Kandarp Dave;Jane Hartung;Michael Gold;Devika Manickam - 通讯作者:
Devika Manickam
Michael Gold的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Michael Gold', 18)}}的其他基金
Liquid Argon Detector R&D with Applications to the LEGEND and CCM Experiments
液氩检测仪 R
- 批准号:
2209129 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Continuing Grant
Lignin Degradation by Phanerochaete Chrysosporium: Biochemical Characterization of Extracellular Peroxidases
黄孢原毛平革菌 (Phanerochaete Chrysosporium) 的木质素降解:细胞外过氧化物酶的生化表征
- 批准号:
9808430 - 财政年份:1998
- 资助金额:
-- - 项目类别:
Continuing Grant
Regulation of Manganese Peroxidase Gene Expression
锰过氧化物酶基因表达的调控
- 批准号:
9723725 - 财政年份:1997
- 资助金额:
-- - 项目类别:
Continuing Grant
Lignin Degradation by Phanerochaete Chrysosporium: Biochemical Characterization of Extracellular Peroxidases
黄孢原毛平革菌 (Phanerochaete Chrysosporium) 的木质素降解:细胞外过氧化物酶的生化表征
- 批准号:
9506338 - 财政年份:1995
- 资助金额:
-- - 项目类别:
Continuing grant
Regulation of Manganese Peroxidase Gene Expression
锰过氧化物酶基因表达的调控
- 批准号:
9405978 - 财政年份:1994
- 资助金额:
-- - 项目类别:
Continuing grant
Lignin Degradation by Phanerochaete chrysophorium: Biochemical Characterization of Extracellular Peroxidases
黄原平革菌降解木质素:细胞外过氧化物酶的生化表征
- 批准号:
9207997 - 财政年份:1992
- 资助金额:
-- - 项目类别:
Continuing grant
Manganese Peroxidase Gene Expression: Regulation by Mn
锰过氧化物酶基因表达:Mn 的调节
- 批准号:
9104610 - 财政年份:1991
- 资助金额:
-- - 项目类别:
Continuing grant
Lignin Degradation by Phanerochaete Chrysosporium: Biochemical Characterization of Extracellular Peroxidases
黄孢原毛平革菌 (Phanerochaete Chrysosporium) 的木质素降解:细胞外过氧化物酶的生化表征
- 批准号:
8607279 - 财政年份:1986
- 资助金额:
-- - 项目类别:
Continuing grant
Lignin Degradation by Phanerochaete Chrysosporium: A Biochemical Genetic Approach
黄孢原毛平革菌的木质素降解:一种生化遗传方法
- 批准号:
8311441 - 财政年份:1983
- 资助金额:
-- - 项目类别:
Continuing grant
Lignin Degradation By Phanerochaete Chrysosporium: a Biochemical Genetic Approach
黄孢原毛平革菌的木质素降解:一种生化遗传方法
- 批准号:
8114218 - 财政年份:1981
- 资助金额:
-- - 项目类别:
Continuing grant
相似海外基金
Molecular mechanism of the PRC-dependent RNA degradation by the rixosome
核糖体PRC依赖性RNA降解的分子机制
- 批准号:
DP240101212 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Discovery Projects
ERI: Degradation of Polyelectrolyte Complexes via Enzyme Addition
ERI:通过添加酶降解聚电解质复合物
- 批准号:
2347080 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
Collaborative Research: RAPID: A perfect storm: will the double-impact of 2023/24 El Nino drought and forest degradation induce a local tipping-point onset in the eastern Amazon?
合作研究:RAPID:一场完美风暴:2023/24厄尔尼诺干旱和森林退化的双重影响是否会导致亚马逊东部地区出现局部临界点?
- 批准号:
2403883 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
RUI: Role of Crystallinity and Morphology in Degradation and Drug Release of PEO-b-PCL Films
RUI:结晶度和形态在 PEO-b-PCL 薄膜降解和药物释放中的作用
- 批准号:
2406566 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
RUI: CAS: Bespoke Polymer Degradation via Topology and Sidechain Variation
RUI:CAS:通过拓扑和侧链变化定制聚合物降解
- 批准号:
2401038 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
Household adaptation amongst hot spots of land degradation vulnerability and bright spots of resilience
土地退化脆弱性热点和复原力亮点中的家庭适应
- 批准号:
2343014 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
Enhancing climate resilience in eastern Africa by co-developing equitable solutions to land degradation and supporting their implementation
通过共同制定土地退化问题的公平解决方案并支持其实施,增强东非的气候适应能力
- 批准号:
NE/Z503447/1 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Research Grant
Visualization of degradation mechanisms and ions migration pathways in perovskite solar cells
钙钛矿太阳能电池中降解机制和离子迁移路径的可视化
- 批准号:
23K26768 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (B)
The origins of complex CMAS deposits in aircraft engines and their effects on engine degradation
飞机发动机中复杂 CMAS 沉积物的起源及其对发动机退化的影响
- 批准号:
2906089 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Studentship
Collaborative Research: RAPID: A perfect storm: will the double-impact of 2023/24 El Nino drought and forest degradation induce a local tipping-point onset in the eastern Amazon?
合作研究:RAPID:一场完美风暴:2023/24厄尔尼诺干旱和森林退化的双重影响是否会导致亚马逊东部地区出现局部临界点?
- 批准号:
2403882 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant