RPG: Molecular Systematic Studies of the n=9 Species in the Genus Passiflora (Passifloraceae)

RPG：西番莲属（西番莲科）n=9 种的分子系统研究

• 批准号: 9209556
• 负责人: James Ackerman
• 金额: $ 2.42万
• 依托单位: University of Puerto Rico-Rio Piedras
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9209556&HistoricalAwards=false
• 关键词: RPG; Molecular; Systematic; Studies; n=9

This project proposes to study the taxonomic relationships among the species of a group of passion flowers. Although the genus Passiflora comprises about 450 New World species, this study will focus on 70 species in the group with a common haploid chromosome number of n = 9. This group contains the majority of the showy- flowered and cultivated species which have edible fruit. The method proposed for this taxonomic study is the comparative analysis of the chlorpolast DNA using restriction site enzymes. Chloroplast DNA is usually inherited only through the female plant, but chloroplasts have been reported to be present in the pollen tubes of one species, so artificial hybrids will be analysed with their known female and pollen parents to test the inheritance pattern of the chloroplast DNA in Passiflora. Nuclear ribosomal DNA will also be used to test field collected species for possible hybrid origin. The data obtained in this study should be of use, not only to shed light on the relationships of this group of passion flowers, but also to elucidate the relationships among a group of Neotropical butterflies in the genus Heliconius whose larvae feed exclusively on the leaves of Passiflora species.

本课题拟对西番莲属植物的种间分类关系进行研究。虽然西番菊属在新世界约有450种，但本研究将重点关注具有共同单倍体染色体数n = 9的群体中的70种。这一组包括大多数艳丽的花和栽培品种，有可食用的果实。提出的分类学研究方法是利用限制性内切位点酶对叶绿体DNA进行比较分析。叶绿体DNA通常只通过雌性植物遗传，但有报道称叶绿体存在于一个物种的花粉管中，因此人工杂交将与已知的雌性和花粉亲本进行分析，以测试西番莲叶绿体DNA的遗传模式。核糖体DNA也将用于测试现场收集的物种可能的杂交起源。本研究结果不仅有助于揭示西番莲属植物的亲缘关系，而且有助于阐明以西番莲属植物叶片为食的新热带蝴蝶属（Heliconius）的亲缘关系。