RPG: Molecular Systematic Studies of the n=9 Species in the Genus Passiflora (Passifloraceae)

本项目拟研究 西番莲属的一种。 虽然属 西番莲包括约450种新世界物种，这项研究将 集中在70个物种中的一个共同的单倍体染色体组 数量n = 9。 这个群体包含了大多数的炫耀- 有可食用果实的花的和栽培的种类。 的 本分类学研究的方法是比较 使用限制性位点酶分析Chlorpolast DNA。 叶绿体DNA通常只通过 雌性植物，但据报道叶绿体存在 在一个物种的花粉管中，所以人工杂交将 与已知的雌性和花粉亲本进行分析，以测试 西番莲叶绿体DNA的遗传模式 核核糖体DNA也将用于测试现场收集的 可能的杂交起源。 在此获得的数据 研究应该是有用的，不仅要阐明 这一组西番莲的关系，但也 阐明了一组新热带区之间的关系 蝶形蝶属的蝴蝶，其幼虫只吃 在西番莲属植物的叶子上。

This project proposes to study the taxonomic relationships among the species of a group of passion flowers. Although the genus Passiflora comprises about 450 New World species, this study will focus on 70 species in the group with a common haploid chromosome number of n = 9. This group contains the majority of the showy- flowered and cultivated species which have edible fruit. The method proposed for this taxonomic study is the comparative analysis of the chlorpolast DNA using restriction site enzymes. Chloroplast DNA is usually inherited only through the female plant, but chloroplasts have been reported to be present in the pollen tubes of one species, so artificial hybrids will be analysed with their known female and pollen parents to test the inheritance pattern of the chloroplast DNA in Passiflora. Nuclear ribosomal DNA will also be used to test field collected species for possible hybrid origin. The data obtained in this study should be of use, not only to shed light on the relationships of this group of passion flowers, but also to elucidate the relationships among a group of Neotropical butterflies in the genus Heliconius whose larvae feed exclusively on the leaves of Passiflora species.