Neural Mechanisms of Ovarian Hormone Action & Lordosis; Studies Using C-FOS

卵巢激素作用的神经机制

• 批准号: 9211307
• 负责人: Janice Thornton
• 金额: $ 1.8万
• 依托单位: Oberlin College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-15 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9211307&HistoricalAwards=false
• 关键词: Neural; Mechanisms; Ovarian; Hormone; Action

Although gonadal hormones act on the brain to influence a variety of processes, it is unclear exactly how these hormones act on the brain to produce their effects. Recently, induction of the proto-oncogene c-fos has been used as a tool to identify individual neurons and areas of the brain which are activated by particular physiological stimuli. The present project proposes to adapt the technique of c-fos immunocytochemistry to examine which nuclei in the brain are activated during exposure to the ovarian hormones estrogen and progesterone and during sexual behavior in the female guinea pig. In addition, the techniques to double label cells for c-fos and a variety of neuropeptides will be developed so that it can be ascertained whether particular neuropeptides are contained in the cells which are activated by ovarian hormones and sexual behavior. This will give us further insights into exactly how and where these gonadal hormones may be exerting their effects and will provide the preliminary data to form the basis of a grant proposal which will examine whether the noradrenergic system and ovarian hormones interact at a particular subset of neurons to exert their facilitatory actions on female lordosis behavior.

虽然性腺激素作用于大脑， 目前还不清楚这些激素是如何作用于这些过程的。 大脑产生影响。 最近， 原癌基因c-fos已被用作识别个体的工具， 神经元和大脑区域被特定的 生理刺激 本项目建议调整 用c-fos免疫细胞化学技术检测 大脑在暴露于卵巢激素时被激活 雌激素和孕激素以及女性性行为期间 豚鼠。 此外，双标记细胞的技术 c-fos和各种神经肽将被开发出来， 可以确定是否含有特定的神经肽 卵巢激素和性激素激活的细胞中， 行为 这将使我们进一步了解究竟如何， 这些性腺激素可能发挥作用， 提供初步数据，作为拨款建议的基础 它将检测去甲肾上腺素能系统和卵巢 激素在特定的神经元子集上相互作用， 对女性脊柱前凸行为的促进作用。