Biochemical and Molecular Evaluation of Low Temperature Acclimation in Ripening Tomato Fruit

成熟番茄果实低温驯化的生化和分子评价

• 批准号: 9213782
• 负责人: Floyd Woods
• 金额: $ 1.3万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-15 至 1994-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9213782&HistoricalAwards=false
• 关键词: Biochemical; Molecular; Evaluation; Low; Temperature

The research will investigate the biochemical and molecular mechanism(s) involved in low temperature acclimation of ripening tomato which include: (1) cell wall composition and associated enzymes, and carbohydrate accumulation; (2) determination of chilling - induced proteins during acclimation in both chilling susceptible and tolerant lines and changes in the transcriptional activity related to acclimation. %%% Chilling injury is a common physiological disorder which occurs upon exposing tropical plants, such as tomato, to low but non- freezing temperatures. Fruits exposed to chilling injury have reduced storage length, as well as quality, due to adverse alterations in ripening process. Acclimation to chilling injury leads to accumulation of new proteins that may be involved in ethylene biosynthesis, cell wall hydrolysis, turnover of pectic fragments, and/or accumulation of carbohydrates which serve as cryoprotectants. The project will emphasize the early stages of acclimation process and mechanisms related to chilling tolerance which are not well characterized.

这项研究将调查生物化学和分子 低温驯化的机制 成熟番茄，包括：（1）细胞壁组成和 相关酶和碳水化合物积累;（2） 驯化过程中冷诱导蛋白的测定 在低温敏感和耐冷品系中， 转录活性与驯化有关。 %%% 冷害是一种常见的生理障碍， 当将热带植物如番茄暴露于低但非低的温度时， 冰冻的温度。 遭受冷害的水果 减少存储长度，以及质量，由于不利的 成熟过程中的变化。 冷驯化 损伤导致新蛋白质的积累， 参与乙烯生物合成，细胞壁水解， 果胶碎片的周转，和/或 作为冷冻保护剂的碳水化合物。 该项目将 强调适应过程的早期阶段， 与耐冷性相关的机制， 表征了