In vivo cloning of defined chromosomal segments into YAC vectors

该项目旨在开发一种技术， 在这些情况下，需要在长图距上进行基因组步行 其中待克隆的基因已经定位在两个限制性内切酶之间， 片段标记在该生物体的RFLP图谱中。 的大小限制 可克隆区域将不由通常的限制来确定 因为这个过程将涉及到靶向的 拟南芥染色体之间的体内遗传重组， 特化酵母染色体（YAC）载体。 中央 一个想法是克隆一个确定的遗传区间的大的物理尺寸 通过细胞融合和体内 同源重组 %%% 这项技术的发展将提高 科学家以拟南芥为模型克隆整个基因组 比现在预计的要快得多

This project seeks to develop technology that will bypass the need for genomic walking over long map distances in those cases where the gene to be cloned has been mapped between two restriction fragment markers in an RFLP map of the organism. The size limit of the clonable region will not be determined by the usual limitations of in vitro cloning because the procedure will involove targeted in vivo genetic recombination between Arabidopsis chromosomes and specialized Yeast Artifical Chromosome (YAC) vectors. The central idea is to clone a defined genetic interval of large physical size in a single step into a YAC vector by cell fusion and in vivo homologous recombination. %%% The development of this technology will enhance the ability of scientists using Arabidopsis as a model to clone the entire genome in much less time than is now predicted.